摘要
目的 :探讨丹肠内营养对家兔肠道的保护作用。方法 :4 4只 10周龄近交系纯种大耳白幼兔 ,随机分为正常对照组、内毒素攻击组、能全力组和丹参肠内营养组 ,实验期为 10 d。实验结束时观察动物的体质量、血清白蛋白、超氧化物歧化酶 (SOD)、丙二醛 (MDA )、内毒素、小肠黏膜可溶性 Ig A、外周血淋巴细胞 DNA损伤等指标的变化。结果 :丹参肠内营养组血清内毒素水平为 (0 .5 3± 0 .2 4 ) Ek U/ L,明显低于内毒素攻击组的(2 .33± 0 .4 1) Ek U/ L(P<0 .0 1) ;而小肠黏膜 s Ig A(1.6 4± 0 .6 5 )较内毒素攻击组 (1.2 5± 0 .2 9)有升高趋势 ;血清 MDA水平为 (0 .5 32± 0 .197) μm ol/ L,也显著低于内毒素攻击组 (0 .890± 0 .0 5 1) μm ol/ L(P<0 .0 1)。与对照组比较 ,丹参肠内营养组家兔外周血淋巴细胞 DNA移动距离显著减小 (P<0 .0 1)。结论 :丹参肠内营养可以通过降低机体氧化损伤 ,保护肠道黏膜细胞 ,维护肠屏障功能 ,减少内毒素的移位 ,从而阻止病程发展 ,减轻器官功能损害。
Objective: To explore the protective effects of salvia miltiorrhiza for enteral nutrition on the gut function in rabbits. Methods: Fortyfour 10week old rabbits were randomly divided into normal control group, lipopolysaccharide(LPS) group, salvia miltiorrhiza for enteral nutrition group and nutrition fibre (group(DS)). The experiment period was 10 days. At the end of experiment, the body weight, plasma (albumin), the superoxide dismutase(SOD) activity and malondialdehyde(MDA) content, endotoxin level and secretory immunoglobubin A(sIgA) in small intestine mucosa and lymphocyte DNA damage were measured. (Results): The serum endotoxin level in salvia miltiorrhiza for enteral nutrition group was (0.53±0.24) EkU/L, it was significantly lower than that of LPS group (2.33±0.41)EkU/L (P<0.01); the contents of sIgA ((1.64±0.65)) had a increasing trend compared with LPS group (1.25±0.29); the serum MDA level was ((0.532±0.197)μmol/L), it was significantly decreased than that of control group(0.890±0.051)μmol/L((P<0.01)); the DNA shift distance was markedly reduced than LPS group(P<0.01). Conclusion: Salvia (miltiorrhiza) for enteral nutrition could prevent gut function damage, protect intestinal mucosa, maintain the (intestinal) barrier function by reducing the oxidative damage and decreasing the LPS translocation.
出处
《中国中西医结合急救杂志》
CAS
2004年第4期227-230,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
天津市卫生局重点科研基金资助项目(01KY17)
关键词
丹参肠内营养
丙二醛
内毒素
DNA损伤
salvia miltiorrhiza
enteral nutrition
malondialdehyde
lipopolysaccharide
DNA damage
