摘要
押目的探讨重组人促红细胞生成素(rHuEPO)对大鼠脊髓损伤后中性粒细胞趋化因子穴CINC-1雪表达的影响。方法SD大鼠102只,随机分为4组,采用改良Allen’s脊髓损伤打击模型,以逆转录-聚合酶链反应穴RT-PCR雪法测定伤段脊髓组织CINC-1mRNA的表达情况。结果正常脊髓组织内存在CINC-1mRNA的表达,脊髓损伤后CINC-1mRNA表达迅速增高,伤后6h达到高峰;rHuEPO治疗组脊髓损伤后6、12小时CINC-1mRNA表达明显低于NS治疗组。结论CINC-1参与继发性脊髓损伤过程,rHuEPO抑制脊髓损伤后CINC-1mRNA的表达,对脊髓继发性损伤可能有保护作用。
Objective To study the effect of recombinant human erythropoietin(rHuEPO) on cytokine-induced neutrophil chemoattractant(CINC-1) gene expression after spinal cord injury(SCI) in rats. Methods 102 SD rats were divided randomly into four groups. The modified Allen's model of spinal cord injury was established. The expression of CINC-1mRNA after SCI was measured by reverse transcription polymerase chain reaction(RT-PCR). Results The expression of CINC-1mRNA was detected in non-injured spinal cord ,quickly up-regulated after SCI, peaked at 6h post-injury. rHuEPO significantly reduced the expression of CINC-1mRNA in the injured cord in comparison to control animals receiving NS vehicle at 6?12h after SCI. Conclusion CINC-1 may play a negative role in the secondary SCI. rHuEPO reduces the expression of CINC-1mRNA in the injured spinal cord,has neuroprotective effect in the secondary SCI.
出处
《颈腰痛杂志》
2004年第4期244-247,共4页
The Journal of Cervicodynia and Lumbodynia
关键词
脊髓损伤
中性粒细胞趋化因子
重组人促红细胞生成素
基因表达
聚合酶链式反应
spinal cord injury
cytokine-induced neutrophil chemoattractant(CINC-1)
recombinant human erythropoietin(rHuEPO)
gene expression
PCR
