摘要
目的运用图像分析技术探讨人脾脏细胞核几何参数变化与死亡时间的关系。方法选31例已知死亡时间的人离体脾组织,在死后36h内每h细胞学涂片、Feulgen-Vans染色,对人脾细胞核的异形指数等5个几何参数进行图像分析。结果脾脏细胞核异形指数在死后5～36h呈上升趋势,r=0.983,线性回归方程y=11.334x-33.339,面积、等效直径、平均直径、周长均与死亡时间无明显的相关性。结论异形指数能较客观地反映人脾细胞核DNA降解后的离散分布状况,与死亡时间有明显相关性,可能是推断早期准确死亡时间的较好形态学指标。
Objective Using computer image-analyze technique(CIAT)to study changes of geometry pa-rameters in human spleen nuclei and seek a new experimental method to deduce the estimation the post-mortem interval(PMI).Methods31cadavers that known accurate PMI,sampled and smeared respectively every hour within the first36hours after death,fixed with cold Carony fixation,stained by Feulgen-van's method,and measured5geometry parameters using the image-analyze instrument including Area(A),Mean-Dia(MD),Average Diameter(AD),perimeter(P),Index of density(ID).Results A,MD,AD and P in the human spleen nuclei have no correlation with the PMI.But ID rose regularly with the prolongation of PMI in36hours.There was a definite correlation between ID and the PMI,r=0.983,linear regression equation with PMI(hours)as the dependent variable was calculated for ID.Conclusion Geometry parame-ter ID was proved to be preferable indexes for estimation of PMI in36hours.
出处
《法医学杂志》
CAS
CSCD
2004年第3期140-142,共3页
Journal of Forensic Medicine
基金
武汉市青年科技晨光计划基金资助项目(20015005049)
