摘要
目的 研究乳腺肿瘤摄取99Tcm 甲氧基异丁基异睛 (99Tcm MIBI)与耐多药蛋白表达水平间的相关性。方法 30例经病理证实的原发性浸润性导管癌患者 ,均行99Tcm MIBI早期显像和延迟显像。对显像阳性的肿块计算感兴趣区与对侧正常相应部位的放射性计数比值 ,以早期摄取比值(EUR)和延迟摄取比值 (DUR)计算滞留指数 (RI)。以免疫组织化学法检测手术切除标本肿瘤组织的P 糖蛋白 (P gp)和多药耐药相关蛋白 (MRP)的表达水平。数据分别进行配对t检验、Pearson相关分析及偏相关分析。结果 P gp的平均表达水平为 0 .1183± 0 .0 70 0 ,MRP的平均表达水平为 0 .1195±0 .0 5 2 2。P gp和MRP的表达水平在RI≥ 0和RI <0的组中 ,差异有显著性。P gp的表达与RI(r=- 0 .919,P =0 .0 0 1)、DUR(r=- 0 .6 75 ,P =0 .0 0 1)和MRP(r=0 .5 4 9,P =0 .0 0 2 )有相关性 ,与EUR(r=- 0 .0 97,P =0 .6 10 )无相关性。MRP的表达与RI(r =- 0 .5 4 7,P =0 .0 0 2 )有相关性 ,与EUR(r=0 .2 92 ,P =0 .117)、DUR(r =- 0 .173,P =0 .36 1)无相关性。P gp与MRP的表达有相关性 (r =0 .5 4 9,P =0 .0 0 1)。偏相关分析则显示 ,P gp的表达与RI(r =- 0 .884 7,P =0 .0 0 1)有显著相关性 ,MRP的表达与RI(r=- 0 .12 96 ,P =0 .5 12 )无相关性。?
Objective To assess the correlation between uptake of 99 Tc m MIBI and expression level of multidrug resistant protein in breast cancer. Methods Thirty pathologically confirmed patients with primary invasive ductal carcinoma were examined by 99 Tc m MIBI scintigraphy at 15 min and 90 min after injecting the tracer. The uptake of 99 Tc m MIBI at the region of interest (ROI) was evaluated as tumor to normal background (T/N) ratio. Retention index (RI) was calculated from the early uptake ratio (EUR) and updelayed take ratio (DUR). The expression of P glycoprotein (P gp) and multidrug resistant associated protein (MRP) was detected by immunohistochemistry and the results were expressed as optical density ( A ). The data were analyzed by t test, Pearson correlation and partial correlation analysis. Results The expression of P gp and MRP in tumor tissue was 0.1183±0.0700 and 0.1195±0.0522, respectively. In group with RI<0. the expression of P gp and MRP was 0.2181±0.0384 and 0.1718±0.0479, respectively. In group with RI≥0, the expression of P gp and MRP was 0.1057±0.0217 and 0.0967±0.0362, respectively. There were significant differences between the two groups ( t =6.61, P =0.0001; t =5.01, P =0.002). There was positive correlation between P gp and RI ( r =-0.919, P =0.001), DUR ( r =-0.675, P =0.001), MRP( r =0.549, P =0.001), respectively, but no correlation between P gp and EUR ( r =-0.097, P =0.610). There was positive correlation between MRP and RI ( r =-0.547 , P =0.002), but no correlation between MRP and EUR ( r =0.292, P =0.117) or DUR ( r =-0.173 , P =0.361). Partial correlation analysis showed that there was high positive correlation between P gp and RI( r =-0.8847, P =0.001), but no significant correlation between MRP and RI( r =-0.1296, P =0.512 ). Conclusion The study suggests that increased level of P gp expression may contribute to a low accumulation of 99 Tc m MIBI in breast cancer, but no correlation with the expression level of MRP. Thus 99 Tc m MIBI scintigraphy may predict the MDR development associated with P gp expression in breast carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2004年第6期353-355,共3页
Chinese Journal of Oncology
