摘要
目的 探讨在食管拉网细胞中进行食管癌基因检测的方法及可行性 ,了解食管癌前期病变细胞中p5 3基因的突变与癌变的关系。方法 对四川省盐亭县 1982年进行食管癌普查的食管拉网脱落细胞涂片标本 ,应用聚合酶链反应 -单链构象多态性分析 (PCR SSCP)方法 ,检测其p5 3基因第 5外显子及第 7外显子的突变情况。结果 全组 4 8例标本中 ,食管正常上皮和重度不典型增生上皮各 2 4例 ,p5 3基因检测均获成功。食管重度不典型增生上皮细胞中有 5例检测到突变 ,均为p5 3基因第 7外显子突变 ,而第 5外显子未检测到突变 ;正常食管鳞状上皮拉网脱落细胞中未发现突变。检测到突变的 5例食管上皮重度不典型增生者 ,有 3例分别在 10年、12年和 14年后转变为食管癌。结论 p5 3基因突变的食管上皮不典型增生细胞具有明显的癌变趋势 ;对食管拉网脱落细胞涂片标本进行微量DNA的提取、扩增和基因检测 ,可作为研究食管癌前期病变的一种新方法。
Objective To explore the feasibility of detecting p53 gene mutation in exfoliative esophageal cells, and compare gene mutation between precancerous lesions and normal esophageal exfoliative cells and correlate p53 gene mutation with esophageal carcinogenesis. Methods Forty eight samples (24 normal squamous epithelia and 24 severe squamous dysplasia) were obtained by balloon cytologic technique from a high incidence area, Yanting county, Sichuan Province, China in 1982. p53 gene mutations in exons 5 and 7 were analyzed by PCR SSCP. Results p53 genes were detected in all samples. Five samples with p53 mutaion were detected in exon 7 and no mutation was detected in exon 5 in 24 severe dysplasia samples. Three of the 5 samples with mutaion in exon 7 developed esophageal cancer in 1992, 1994 and 1996 respectively. No p53 gene mutation was detected in exon 5 and 7 in normal exfoliative samples. Conclusion p53 mutation may have occurred in the precancerous lesions which contributes in the initiation of human esophageal carcinogenesis.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2004年第6期349-352,共4页
Chinese Journal of Oncology
