摘要
目的 :观察尿中乳果糖 /甘露醇能否反映肠黏膜的通透性。 方法 :建立用高效液相色谱 示差检测器测定尿中乳果糖、甘露醇含量的方法。尿样经过超滤、离子交换处理后在LichrosherNH2 柱 (4 .6mm× 2 5 0mm ,5 μm)上 ,以乙腈、水 (体积比 72∶2 8)为流动相 ,示差检测器检测。 结果 :乳果糖、甘露醇的回收率分别为 (97.86±3.5 5 ) %与 (99.1 9± 3.1 4 ) % ,相对标准偏差分别为 2 .1 2 %与 2 .0 4 %。测定 1 8例正常人口服乳果糖 1 0g和甘露醇5g ,6h后测定尿中乳果糖、甘露醇含量 ,乳果糖 /甘露醇比值为 (0 .0 2 75± 0 .0 1 1 3)。 结论 :尿中乳果糖
Objective: Lactulose and mannitol excretion ratio in urine appears to be a marker to measure the intestinal permeability. Methods: A method has been developed for the simultaneous determination of lactulose and mannitol in urine by high performance liquid chromatography.After ultrafiltered and deionized,urine samples were analyzed with a lichrosher NH 2 column(4.6 mm×250 mm,i.d.,5 μm),a mix of acetonitril water (72∶28,volume ratio) as mobile phase and refractive index detector. Results: The recoveries were 97.86%、 99.19% respectively and the relative standard deviation were 2.12、2.04.In 18 urine samples from healthy volunteers and patients who ingested the test solution containing 10 g lactulose and 5 g mannitol,the 6 h urinary excretion ratio of lactulose/mannitol was 0.0275±0.0113. Conclusions: It is suggested that lactulose and mannitol excretion ratio was anaccurate marker of intestinal permeability.
出处
《肠外与肠内营养》
CAS
2004年第4期237-238,共2页
Parenteral & Enteral Nutrition
