摘要
目的 测定根据计算机预测的SARS冠状病毒S蛋白S1区的抗原表位多肽与SARS病人血清的免疫反应,以寻找和确定SARS相关冠状病毒的中和抗原表位。方法 采用多肽合成仪合成SARS冠状病毒S蛋白S1区的多肽,并用ELISA方法检测合成的多肽与SARS病人血清的免疫反应。结果 合成的8个多肽与SARS病人血清免疫反应的光密度值是其与正常人血清免疫反应的光密度值的1.5～2.6倍。结论 合成的多肽与SARS病人血清的免疫反应较低,提示这些多肽是否为SARS冠状病毒的抗原表位还要进一步研究。
Objective To examine the immunological reactions between the peptides of SARS coronavirus (SARS-Cov) S-protein and the serum of SARS patients for identifying the SARS-Cov epitope. Methods The peptides from S1 domain of SARS-Cov S-protein were synthesized by peptide synthesizer, and the immunological reaction of the peptides with the serum of SARS patients were examined by means of ELISA. Results The optical density value of the immunological reaction of synthesized 8 peptides with SARS patient serum was 1.5-2.6 times higher than that with normal serum. Conclusion The 8 peptides from S1 domain of S protein appear to have low immunogenicity to the serum of SARS patients, indicating that these peptides may not be the epitope of the SARS-Cov.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第7期789-790,797,共3页
Journal of First Military Medical University
基金
广东省科技厅科研攻关项目SARS专项基金~~
关键词
冠状病毒
SARS
表位
多肽疫苗
免疫反应
coronavirus, severe acute respiratory syndrome
epitope
peptide vaccine
immune reaction
