摘要
目的 探讨Celecoxib(塞来昔布 )诱导C6胶质瘤细胞凋亡的作用。方法 应用吖啶橙、透射电镜和流式细胞仪 (FCM)检测方法。结果 通过吖啶橙及电镜检查发现Celecoxib 5 0 μmol/L组细胞凋亡明显多于12 .5 μmol/L组 ,FCM检测Celecoxib 5 0 μmol/L组细胞凋亡为 15 .32 % ,12 .5 μmol/L组凋亡为 5 .83% ,不同浓度的Cele coxib对C6胶质瘤细胞有增殖抑制及诱导凋亡的作用 ,凋亡随药物浓度增加而升高。结论 Celecoxib对C6胶质瘤细胞呈剂量依赖性抑制及诱导凋亡的作用 ,对胶质瘤治疗有重要意义。
Objective To investigate celecoxib’s function of inducing C 6 glioma cells apoptosis in vitro. Methods Cell apoptosis was evaluated by acridine orange(AO),electron microscope and flow cytometry(FCM). Results From AO and electron microscope we could see apoptosis number of C 6 glioma cells depending on concentration of celecoxib,FCM found apoptosis of celecoxib 50μmol/L and 12.5μmol/L group were 15.32%and 5.83%,respectively. Celecoxib could increase apoptosis in vitro,there was a dose-dependent tendency. Conclusion Celecoxib can induce apoptosis of C 6 glioma cells.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2004年第3期243-244,i002,共3页
Journal of Apoplexy and Nervous Diseases
