摘要
目的 探讨适合中国东北地区的特异性抗体的检测方法、阳性标准及临床意义。方法 应用酶联免疫吸附试验 (ELISA)方法检测抗原物质特异性抗体 ,摸索反应条件。按确定的最适条件对中国东北地区 5 8例哮喘病人及4 7例健康对照者血清中特异性抗体进行检测。结果 确定了ELISA方法的最适包被浓度 ,最适反应浓度 ,和反应时间及温度。以OD值≥健康对照组平均OD值的 1 5倍作为阳性标准 ,发现本地区哮喘病人血清中对蒿草花粉、春季花粉、淡水鱼、蒜这 4种抗原物质的特异性抗体阳性率高于正常对照组 (P <0 0 5 )。结论 可以将以上改良的ELISA方法和阳性标准应用于本地区的哮喘病人过敏原的检测。
Objective To approach allergen-specific antibody detection method and diagnosis standard that suit Chinese northeast area,and clinical significance. Methods We searched for every reaction condition of Enzyme linked immunosorbent assay(ELISA), by which we detected antigen-specific antibodies. According to the definited most suitable reaction conditions, we detected the antigen-specific antibodies in serum of 58 patients with bronchial asthma and 47 normal control subjects in Chinese northeast area. Results According to the definited most suitable reaction conditions, using OD≥1.5 times of the average OD of normal control subjects as positive standard, we found that the positive ratio of specific antibodies of four antigens, including artemisia family pollen, spring pollen, fresh water fish and garlic, were higher in asthmatic patients than in control(P<0.05). Conclusion we can detect allergens in asthmatic patients by above improved ELISA and positive standard in Chinese northeast area.
出处
《中国实验诊断学》
2004年第3期276-278,共3页
Chinese Journal of Laboratory Diagnosis
关键词
过敏原特异性抗体
检测方法
阳性标准
allergen-specific antibody
detection method
positivity standard
