摘要
目的 观察光气致肺水肿小鼠肺Ⅱ型细胞发生凋亡情况和血清、肺脏的血管内皮生长因子 (VEGF)、VEGF受体 (Flt1)的变化及肺脏VEGFmRNA的表达。方法 2 6只二级BALB C小鼠 ,雄性 ,随机分为 2组 :对照组和染毒组 (各 13只 )。对照组小鼠以空气为对照 ,染毒组小鼠给予 11.9mg L剂量的光气 ,时间均为 5min ,染毒后 4h ,分离小鼠原代肺Ⅱ型细胞 ,电镜观察两组小鼠肺Ⅱ型细胞凋亡情况 ,酶联免疫法测定血清和肺脏的VEGF、Flt1的含量及反转录PCR法测定肺脏VEGFmRNA的表达。结果 电镜显示光气染毒肺水肿小鼠原代肺Ⅱ型细胞出现凋亡小体 ;染毒小鼠血清VEGF和肺脏的VEGF、Flt1含量 [(134.0 7± 12 0 .2 6 )、(4 77.76± 98.0 6 )、(12 818.4 8± 2 30 4 .15 )pg ml]明显低于对照组 [(4 4 5 .5 7± 173.30 )、(10 2 6 .87± 4 74 .5 6 )、(2 1976 .5 1± 74 2 1.0 1)pg ml],差异有显著性 (P <0 .0 5 ) ;染毒小鼠血清Flt1含量 [(2 36 9.5 6± 381.70 )pg ml]明显高于对照组 [(1898.0 0± 4 5 3.6 9)pg ml],差异有显著性 (P <0 .0 5 ) ;染毒小鼠肺脏VEGFmRNA表达降低。结论 光气经呼吸道染毒可引起肺水肿小鼠原代肺Ⅱ型细胞发生凋亡 ,使血清VEGF和肺脏VEGF、Flt1降低及肺脏VEGFmRNA表达降低。
Objective To study the apoptosis of alveolar type Ⅱ cells,alterations of vascular endothelial growth factor(VEGF),VEGF receptor(Flt1) in serum and lung and expression of VEGF mRNA in lung in pulmonary edema mice induced by phosgene. Methods Twenty-six BALB/C mice were randomly divided into 2 groups:control group,exposed group(13 mice in each group).Mice of exposed group were intoxicated by inhalation of phosgene 11.9 mg/L for 5 minutes.Mice of control group were treated as the same way by inhalation of air.Isolation of mice alveolus type Ⅱ cells 4 h after intoxication was carried out to observe their apoptosis under electron microscope.Contents of VEGF and Flt1 in lung and serum by ELISA,and expression of VEGF mRNA were determined. Results Alveolar type Ⅱ cells were identified by tannic acid stainning and electron microscopy.After exposed to 11.9 mg/L of phosgene for 5 minutes,the apoptotic body in alveolus type Ⅱcells was found in exposed group.The contents of VEGF in serum and lung and Flt1 in lung of exposed mice [(134.07±120.26),(477.76±98.06),( 1 2818.48 ±2 304.15)pg/ml]were significantly lower than those of control group[(445.57± 173.30), (1 026.87±474.56),(21 976.51±7 421.01)pg/ml,P<0.05] but the content of Flt1 in serum[ (2 369.56 ±381.70) pg/ml ] was higher than that in control group[(1 898.00±453.69)pg/ml,P<0.05].The expression of VEGF mRNA in pulmonary edema mice was decreased. Conclusion Phosgene can induce apoptosis of alveolar type Ⅱcells,and decrease in the content of VEGF and Flt1,and expression of VEGF mRNA in lung.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2004年第3期197-199,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
军队十五指令课题 ( 0 1L0 77)
