摘要
目的 :探讨喉咽鳞状细胞癌 (LPSCC)D9S171微卫星位点杂合性缺失 (LOH)的临床意义及对检测颈淋巴结微转移的应用价值。方法 :应用聚合酶链反应对 18例LPSCC及 72个颈淋巴结在 9号染色体上的D9S171微卫星位点进行扩增 ,分析D9S171微卫星位点的DNA等位基因的LOH。结果 :颈淋巴结组织D9S171位点的LOH发生率 4 0 .2 8%显著高于常规病检的阳性率 8.33% (P <0 .0 1) ;肿瘤组织中的LOH发生率与肿瘤分化程度有关 (P <0 .0 5 ) ,并且与肿瘤临床分期呈密切线性正相关性 (P <0 .0 1)。结论 :D9S171位点的LOH分析可能是检测LPSCC颈淋巴结微转移的较为敏感的手段之一 ,并可能作为判断LPSCC患者预后的指标之一。
Objective:To access prognosis and explore neck lymph nodes micrometastasis in larynx and pharynx squamous cell carcinoma (LPSCC) with loss of heterozygosity (LOH).Method:By polymerase chain reaction (PCR), 18 cases of LPSCC and 72 resected neck lymph nodes were investigated with LOH at D9S171 locus.Result:The higher level of LOH ( 40.28 %) was found in neck lymph nodes compared with the positive level of neck lymph nodes detected by pathological examination ( 8.33 %) (P< 0.01 ); LOH was related with tumor differentiation in patients with LPSCC (P< 0.05 ). The LOH rate of primary tumor was closely correlated with the clinical stages (P< 0.01 ).Conclusion:Our results suggested that analysis of LOH at D9S171 locus is one of sensitive means of evaluating neck lymph node micrometastasis, and which may be one of prognostic factors in patients with LPSCC.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2004年第6期321-323,共3页
Journal of Clinical Otorhinolaryngology
关键词
喉咽肿瘤
淋巴转移
DNA
微卫星
杂合性缺失
Larynx and pharynx neoplasms
Lymphatic metastasis
DNA,microsatellite
Loss of heterozygo-sity
