摘要
目的 观察内毒素 /脂多糖 (LPS)致伤大鼠的血浆白细胞介素 (IL)13含量及肺组织激活剂蛋白 1(AP 1)活性的变化 ,探讨AP 1与IL 13表达的关系。 方法 将 12 0只Wistar大鼠根据注射LPS剂量不同随机分为A(2mg/kg)、B(4mg/kg)、C(6mg/kg)、D(8mg/kg)组 ,并设立相应的等渗盐水对照组 (NS组 ) ,取伤后 1、2、4、6h为观察时相点 ,每组每时相点 6只大鼠。致伤Wistar大鼠 ,复制全身炎症反应综合征 急性肺损伤 (SIRS ALI)模型。在致伤后 1、2、4、6h,采用酶联免疫吸附测定法 (ELISA)检测血浆IL 13含量 ,采用凝胶迁移率分析法 (EMSA)检测肺组织中AP 1活性。 结果 LPS可使IL 13含量及AP 1活性同步升高。A、B、C、D组与NS组比较 ,差异均有显著性意义及非常显著性意义 (P <0.0 5～ 0.0 1)。C、D组的血浆IL 13含量及肺组织中的AP 1活性均显著增加 ,D组两指标的峰值分别为 (45 .6 2± 5 .78) pg/ml、(177.6 8± 6.81)NII%,均出现在伤后 2h。 结论 IL 13表达增强时AP 1活性亦升高 ,并与SIRS ALI发生有关。
Objective To investigate the changes in plasma level of interleukin-13 (IL-13) and the changes in the pulmonary IL-13 mRNA content and the pulmonary activator protein-1(AP-1) activity of the rats inflicted with acute lung injury(ALI) induced by lipopolysaccharide (LPS), so as to explore the relationship between IL-13 expression and AP-1 activity. Methods One hundred and twenty Wistar rats were employed in the study and were randomly divided into A (2 mg/kg), B (4 mg/kg), C (6 mg/kg) and D (8 mg/kg) groups according to different dosage of LPS administration and a control group (NS group) at each observing time point. The rats were observed at 1, 2, 4 and 6 postburn hours (PBHs) and every 6 rats were deployed in every group and each time points. A model of systemic inflammatory response syndrome-acute lung injury (SIRS-ALI) was replicated in Wistar rats. The plasma content of IL-13 was assayed by enzyme-linked immunosorbent assay (ELISA),and the pulmonary tissue content of IL-13 mRNA and AP-1 activity by reverse transcriptase-polymerase chain reaction (RT-PCR) and electrophoretic mobility shift assays (EMSA). Results The plasma content of IL-13, pulmonary content of IL-13 mRNA and AP-1 activity increased simultaneously after LPS administration. All the above indices were significantly different statistically between the LPS groups and the control group ( P < 0.05-0.01). The plasma level of IL-13 and pulmonary tissue mRNA content and AP-1 activity in A, B , C and D groups were increased significantly with peak levels at 2 PBHs. Conclusion The pulmonary AP-1 activity increased with the enhanced expression of IL-13, which was related to the development of SIRS-ALI.
出处
《中华烧伤杂志》
CAS
CSCD
2004年第3期148-150,共3页
Chinese Journal of Burns
基金
国家自然科学基金资助项目 (3 973 0 2 10 )
全军医学科学技术研究"十五"重点课题资助项目 (0 1Z0 76)
