摘要
目的:探讨CHB患儿外周血淋巴细胞各亚群的特点及与肝病府情的关系. 方法:采用流式细胞术对CHB患儿29例、正常儿童15例的外周血纯真T细胞亚群、功能T细胞亚群、激活T细胞亚群及凋亡T细胞亚群进行检测,并结合血清转氨酶(ALT) 和病毒载量水平进行统计学分析. 结果:CHB患儿外周血CD4+CD45RA+T,CD4+CD45RO+T, CD4+CD28+T,CD4+HLA-DR+T,CD4+CD25+T, CD4+CD95+T,CD8+HLA-DR+T,CD3+,CD3+CD4+ T,CD3+CD8+T,CD4+/CD8+与正常儿童相应的细胞亚群比较,无统计学差异(P>0.05),但CD8+CD28+T(0.62±0.2 5×109/L),CD8+CD38+T(0.74+0.3 1×109/L), CD8+CD95+T(0.16±0.1 3×109/L)细胞亚群的绝对数与正常对照组CD8+CD28+T(0.44±0.15×109/L),CD8+CD38+T(0. 57±0.1 6×109/L),CD8+CD95+T(0.03±0.01×109/L)比较, 有统计学差异(P<0.05),CD8+CD28+T,CD8+CD38+T 及CD8+CD95+T的构成比同样高于健康的儿童. 结论:CHB患儿外周血CD4+T细胞亚群数量上改变不明显,主要是CD8+T淋巴细胞亚群的异常,其中CD8+CD38+ T.CD8+CD95+T,CD8+CD28+T细胞亚群细胞绝对数较正常儿童明显升高.
AIM: To investigate the characteristics of T-lymphocytic subsets in HBV infected pediatric subjects for evaluation of their clinical implication. METHODS: Fresh peripheral blood samples were obtained from 29 HBV-infected pediatric cases and 15 healthy counterparts. CD4+ and CD8+ T lymphocyte subsets including the naive (CD45RA+), memory (CD45RO+), functional (CD28+), activated (HLA-DR+CD25+) and apoptosis (CD95+) T lymphocytes, respectively, were analyzed by flow cytometry. The clinical data such as serum ALT level, HBV viral load and the number of peripheral lymphocytes were simultaneously recorded from each HBV carrier. RESULTS: HBV-infected children had an obviously increasing percentage of CD8+CD28+T cells (72±11%), CD8+CD38+ (85±6%) and CD8+CD95+(19±12%) T-lymphocytes, and there were significant differences between the children with chronic B hepatitis and the healthy children (P<0.05). As compared to the absolute number of CD8+CD28+(0.62± 0.2 5×109/L), CD8+CD38+(0.74±0.3 1×109/L) and CD8+ CD95+(0.16±0.1 3×109/L) T-lymphocytes, the patients had higher numbers than the healthy children (P <0.05). The increase of numbers of circulating CD8+CD38+, CD8+ CD95+and CD8+CD28+ T-lymphocytes was significant. Furthermore, the association was not found between serum HBV viral load level and the T-lymphocyte subsets. CONCLUSION: Our data may provide valuable information for evaluation of disease progression of HBV infected children
出处
《世界华人消化杂志》
CAS
2004年第6期1295-1298,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助
No.30271230~~
