摘要
根据Y染色体序列特点,含有Y染色体特异重复DNA族(DYZ1)800～5000拷贝、新设计一对引物Y3、Y4经聚合酶链反应(PCR)扩增该序列的特定片段,来检测孕妇血中胎儿细胞。作者用此方法扩增各妊娠早、中、晚孕妇外周血标本粗提DNA,检测男性胎儿细胞,与相应的绒毛、羊水及娩出胎儿性别相比较,符合率分别为93％、100％、87.5％。本研究为无创伤性产前诊断提供了新的途径。
According to that Y-chromosomal sequence in characterised by Y-specific repeat DNA family (DYZ1 ), which contain 800-5000 copies, a pair of primers Y3, Y4 is designed to amplify their 446bp long of specific DNA segment by polymerase chain reaction (PCR), so as to detect male fetal cells in materal blood. In this paper male fetal cells in maternal blood can be detected by PCR amplification of unpurified DNA from maternal peripheral blood during various stages of gestation (early, middle, late). Compared with villi, ammotic fluid and deliverd neonate sex their coincident rate are 93%. 100%, 87. 5% respectively among three periods. It is revealed that noninvasive examining fetal cells from peripheral blood of pregnant women for diagnosis of sex-linked inherited diseases is significant valuable.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1993年第5期418-423,共6页
Academic Journal of Second Military Medical University
关键词
聚合酶链反应
胎儿
细胞
产前诊断
polymerase chain reaction
fetal cells
prenatal diagnosis
