摘要
本文报告了用气相色谱(GC)法测定人体血浆中芬太尼含量的方法。以Ro21-2212作内标,芬太尼的同系物作载体,在碱性条件下用环己烷-异戊醇(197:3)提取,药物反提至硫酸溶液中,碱化后再用乙醚-二氯甲烷(9:1)提取。样品存柱温为255℃的HP-1大口径毛细管柱(10m×0.53mm×2.65μm)上,以氮气作载气和氮磷检测器进行分析。住0.25～100ng/ml范围内有良好线性,最低检测限为0.2ng/ml。平均回收率为99.02％±6.81％,日内和日间变异系数均小于8.0％。方法重现性好、专一性强、灵敏度高,内源性物质、代谢产物及同时使用的药物均不干扰。用本法测定了全麻择期手术病人的药代动力学参数。
A GC method is described for measuring fentanyl in man plasma. Fentanyl was extracted at basic pH with cyclohexane-isobutyl alcohol (197:3) with Ro21-2212 added as internal standard and fentanyl derivative as earner. The drug was back-extracted in H2SO4, then the extract was made basic and recxtracted with ethyl ether-dichloromethane (9:1). The organic phase was evaporated at 40℃ under N2 and the residue was dissolved in ethanol. A. portion (2μl) was analyzed on a wide-bore capillary column (10 m × 0.53 mm) of HP-1 (2.65 μm), operated at 255℃ with N2 as carrier gas (8 ml/min) and N -P detector. The calibration graph was linear within the ranges of 0.25-100 ng/ml with a corelation coefficient of 0.9996 and the detected limit was 0.2 ng/ml. Mean recovery was 99.02% ± 6.81%. The coefficient of variation for the within- and between-run were all less than 8%. No interference was found from endogenous compounds, metabolites of parent drug or other commonly used drugs. The method was applied to therapeutic drug monitoring and pharmacokinetic studies.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1993年第6期517-520,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
