摘要
用^(32)P标记的热休克蛋白70(hsp70)cDNA为探针,检测了热应激时完整大鼠及人骨肉瘤细胞(HOS-8603)的hsp70 mRNA。结果表明,大鼠体温在42℃ 30min后,脑、肝、脾中hsp70 mRNA的表达明显增加,以脑最为显著。HOS-8603细胞经42℃热处理30min,置37℃培养2～4 h后,hsp70 mRNA的表达亦明显增加。表明热应激不论在整体动物还是在离体细胞都可诱导热休克蛋白70mRNA的表达,且在整体动物上有组织特异性。
The induction of hsp70 mRNA in human osteosarcoma cells (HOS-8603) and intacl rats under heat stress was studied using hsp70 cDNA labeled with a-32P dATP. The results showed that the induction of hsp70 mRNA was evident in liver, lung, spleen and the most evident was found in brain when the core body temperature of rats was brought to 42℃ for 15 min. The induction of hsp70 mRNA in HOS-8603 was also significant when cells were cultured at 42℃ for 30 min. These results indicated that hsp70 mRNA could be induced both in vitro and in vivo under heat stress condition and the induction of hsp70 mRNA in intact rats was of tissue-specific fashion.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1993年第6期535-539,共5页
Academic Journal of Second Military Medical University
关键词
热休克蛋白
分子杂交
CDNA
MRNA
heat shock protein
heat stress
molecular hybridization
osteosarcoma cells
cDNA
mRNA
rats
