摘要
以 2年生胡杨实生苗为研究材料 ,通过用 119mmol L的NaCl溶液浇灌处理 1h ,分别收集NaCl处理前后的细嫩白根设立对照样品与处理样品 ,分离mRNA。用抑制性扣除杂交技术建立胡杨盐处理的cDNA扣除文库 ,并以cDNA阵列杂交技术用32 P标记探针进行杂交 ,共筛选 6 72个有效克隆 ,从中确认 3个探针进行了序列分析 ,其中 1个为盐抑制表达基因片段 ,2个为盐诱导表达基因片段。分析表明 ,1个盐诱导表达基因片段为 1含 188氨基酸开放阅读框的EST ,该EST具有 10 79个核苷酸。该结果与我们早先检测到的
Collected 2 year old Populus euphratica tender roots before and 1h after watered 119mmol/L NaCl. Extracted its mRNA to construct salt-related cDNA subtractive library by suppressed subtractive hybridization. Through hybridized with probes labeled ^(32)P, 672 clones had been screened and confirmed 1 of them was salt-suppressed gene segment and 2 were salt-inducible. Analysis shows a salt-suppressed segment is EST contained 188 amino acids opening reading frame, this result is in accordance with our earlier study on P. euphratica salt-inducible proteins. The salt-inducible gene EST is bankited to GenBank with accession number AF315118.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2004年第2期76-80,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金 ( 30 170 76 3)
