摘要
观察急性冠状动脉综合征患者血小板一氧化氮合酶活性及表达改变情况。急性冠状动脉综合征患者32例 ,以健康成人 2 2例作对照 ,取外周静脉血后 ,利用凝胶色谱柱法收集血小板 ,用同位素两步色谱法测定一氧化氮合酶活性 ;蛋白免疫印迹增强化学发光法检测内皮型一氧化氮合酶表达水平。结果发现 ,基础状态下血小板一氧化氮合酶活性正常人为 95 6 .0± 4 8.2pmol 10 8血小板 ,急性冠状动脉综合征患者为 5 2 5 .5± 6 0 .8pmol 10 8血小板 ,明显低于正常人 (P <0 .0 1) ,组胺刺激后血小板一氧化氮合酶活性仍低于正常对照组 (P <0 .0 1) ;与正常人相比 ,急性冠状动脉综合征患者血小板内皮型一氧化氮合酶表达无明显改变。结果提示 ,急性冠状动脉综合征患者血小板一氧化氮合酶活性降低 ,一氧化氮释放减少可能是其血小板活化的机制之一。由于血小板粘附 ,聚集白色血栓形成是急性冠状动脉综合征早期事件 ,这对我们认识冠心病发病机理、开发临床新药提供新的思路。
Aim To observe the activity and expression of platelets nitric oxide synthase (NOS) in patients with acute coronary syndrome (ACS). Methods Peripheral venous blood was collected from both normals and ACS patients. Sepharose CL-2B gel colomn were used to isolate platelets. Nitric oxide synthase (NOS) activity in platelets was assessed by measuring the conversion of 3 H-L-arginine to 3 H-L-citrulline and determined in the absence or presence of histamine or L-NAME; The levels of endothelial NOS (eNOS) expression were measured by western blotting. Results Platelet NOS activity was significantly decreased in ACS patients compared with normal subjects (P<0.01); it was still lower after histamine stimulation (P<0.01); There were no significant change of eNOS expression between ACS patients and normal subjects. Conclusions Platelets NOS activity of ACS patients reduced. Since platelet aggregation and thrombus formation are implicated in unstable angina and myocardial infarction, impaired platelet NOS activity and thus decreased NO production may contribute to development of ACS.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第2期209-211,共3页
Chinese Journal of Arteriosclerosis
