摘要
目的 表达人腺病毒 3型六邻体蛋白 ,为制备基因工程疫苗打下基础。方法 BamHⅠ和SalⅠ双酶切重组克隆质粒pUC19Hexon和载体pQE31,将得到的目的片段定向连接到表达载体pQE31中 ,对目的片段进行DNA测序鉴定 ,IPTG诱导高效表达。结果 构建了表达重组质粒pQE31Hexon ,测序鉴定了克隆序列的准确性。在大肠杆菌中成功地表达了Ad3六邻体蛋白。结论 成功表达了Ad3的六邻体蛋白。
Objective To construct expression recombinant plasmid containing the Hexon gene of Ad3 and express the protein to provide the foundation to produce genetic engineering vaccine.Methods Recombinant plasmid pUC19Hexon and vector pQE31 were purified and cut by restriction enzyme BamHⅠ and SalⅠ. The target fragment was then connected with vector pQE31 by ligase T4. Then expressing recombinant plasmid pQE31 Hexon was charactered by restriction enzyme digestion and sequencing. The fusion protein is expressed in E.coli M15. Results The expressing recombinant pQE31 Hexon was obtained.Meanwhile, it was demonstrated that the expressing fragment was Ad3 Hexon sequence indeed by aligning with Genebank. With the induction by IPTG, the target protein was expressed in E.coli.Conclusion The Ad3 Hexon protein is expressed in E.coli successfully.
出处
《哈尔滨医科大学学报》
CAS
2004年第3期218-220,共3页
Journal of Harbin Medical University
基金
中国医学科学院青年基金资助项目 (93 3 10 14 )
关键词
3型腺病毒
六邻体
表达
重组质粒
adenovirus type 3
Hexon
expressing
recombinant plasmid
