摘要
为了明确红光和蓝光对葡萄叶片衰老与活性氧代谢的影响,以设施延迟栽培条件下叶片衰老速度不同的两个葡萄品种‘意大利’和‘无核白鸡心’为试材,分别进行补充红光和蓝光处理,以未补光处理作对照,研究其对功能叶片衰老期间叶绿素和可溶性蛋白质含量,净光合速率,丙二醛(MDA)含量,过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及超氧阴离子自由基()和过氧化氢(H2O2)含量的影响。研究结果表明:与对照相比,红光明显减缓了叶片中叶绿素和可溶性蛋白质含量以及净光合速率的下降,提高了CAT和SOD活性,减慢了MDA、、H2O2的上升速率,推迟了H2O2出现最大含量的时间,延缓了叶片衰老;蓝光处理下,叶片衰老前期的叶绿素含量、净光合速率、抗氧化酶活性均低于对照,加速了叶片的衰老进程,但在后期叶绿素含量、净光合速率和抗氧化酶活性逐渐高于对照,在一定程度上延缓了叶片衰老。两个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。
To investigate the effects of red light and blue light on leaf senescence and active oxygen metabolism,the two grape variety Vitis vinifera‘Italia'and V. vinifera‘Centenial Seedless' with different senescence-types cultured in protected and delayed cultivation were studied.‘Italia'and‘Centenial Seedless' were exposed to red light and blue light,and no supplemental lighting as control,and the physiological indexes of their leaves,such as chlorophyll and soluble protein contents,the net photosynthetic rate,superoxide radical()and hydrogen peroxide(H2O2)contents,malondialdehyde(MDA)accumulation,superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities were determined during natural senescence. Compared with control,red light delayed the decrease of chlorophyll and soluble protein contents and the net photosynthetic rate significantly,increased the CAT and SOD activities,decreased the MDA, and H2O2 contents,delayed the appearance time of maximum content of H2O2 and postponed the senescence of functional leaves;While blue light significantly decreased the chlorophyll content,the net photosynthetic rate and activity of antioxidant enzymes at early stages of leaf senescence,accelerating the senescence process,after that the chlorophyll content,the net photosynthetic rate and activity of antioxidant enzymes in the blue light treatment were gradually higher than that in the control,so blue light could delay leaf senescence to a certain extent at late sampling stage. There were differences in different senescence-types of grape leaves,the leaves of‘Italia'aged slower and maintained higher antioxidant enzymes activity at the late growth stage.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第6期1066-1076,共11页
Acta Horticulturae Sinica
基金
国家现代农业产业技术体系建设专项资金项目(GARS-20-zp-1)
‘948’重点项目(2011-G28)
国家自然科学基金项目
中国农业科学院创新工程项目(CAAS-ASTIP-2015-RIP-04)
关键词
葡萄
叶片衰老
红光和蓝光
活性氧
代谢
grape
leaf senescence
red light and blue light
reactive oxygen
metabolism
