摘要
目的 探讨不同食管病变组织中环氧合酶-2(COX-2)、CD44v6、增生细胞核抗原(PCNA)的表达情况及其临床表达特点,进一步了解其生物学意义.方法 采集山西省肿瘤医院病理科2003年至2005年手术切除标本并经病理证实的食管鳞状细胞癌(含有上皮内瘤变以及正常食管鳞状上皮组织)标本105例,用免疫组织化学SP法对组织中COX-2、CD44v6、PCNA蛋白进行检测.结果 在105例食管鳞状细胞癌组织中,COX-2、CD44v6、PCNA蛋白的表达随着食管病变的加重而增加(P＜0.05).COX-2与食管癌分化程度和有无淋巴结转移相关.CD44v6在有淋巴结转移的食管癌组织中的表达明显高于无淋巴结转移者.COX-2的表达与CD44v6的表达有相关性.结论 COX-2、CD44v6以及PCNA蛋白的高表达与食管癌的发生有关.COX-2和CD44v6的表达情况可以作为判断食管癌预后的指标.在食管癌组织中COX-2的表达与CD44v6的表达呈正相关.
Objective To examine the expression of COX-2, PCNA, CD44v6 protein in different procancerous lesion and esophageal squamous cell carcinoma tissue, and to investigate the relationship between their expression and the biological level,lymph node metastasis in esophageal squamous cell carcinoma tissue. Methods Immunohistochemistry was demonstrated to show these tumor markers expression in different procancerous lesions and esophageal squamous cell carcinoma tissue. Results Stepwise over-expression of COX-2, CD44v6, PCNA protein were observed in the multi-step process of esophageal tissue. Significant difference in expression of these tumor markers(P ＜0.05) was observed between normal esophageal epithelia and dysplasia. The expression of COX-2 (P ＜0.05) had statistical different in biological level and lymph node metastasis.The expression of CD44v6 (P ＜0.05)had statistical different in lymph node metastasis. The relationship between COX-2 and CD44v6 was statistical significant in esophageal squamous cell carcinoma tissue(P ＜0.01). Conclusion Stepwise over-expression of COX-2,CD44v6 PCNA protein have relation with esophageal squamous cell carcinoma. The overexpression of COX-2 and CD44v6 have effect on prognosis of esophageal squamous cell carcinoma. The expression of COX-2 have relationship with that of CD44v6 in esophageal squamous cell carcinoma.
出处
《肿瘤研究与临床》
CAS
2008年第7期465-467,共3页
Cancer Research and Clinic
