摘要
目的探讨天麻素(Gastrodine,Gas)对大鼠局灶性脑缺血损伤的保护作用及机制。方法线栓法制备大鼠大脑中动脉栓塞模型,缺血2 h后再灌注。天麻素(5,10,20 mg/kg)再灌注同时腹腔给药,再灌注后24 h,测定大鼠神经功能缺失评分和脑梗死体积,伊文思蓝法观察血脑屏障破坏程度,Western blot检测梗死半暗带皮层组织炎症因子TNF-α、IL-1β和IL-6的蛋白表达。结果天麻素(10,20 mg/kg)能改善大鼠脑缺血后神经功能缺失,减小脑梗死体积,降低血脑屏障的通透性。天麻素(20 mg/kg)对梗死半暗带皮层组织TNF-α表达的抑制率为24%,对IL-1β表达的抑制率为34%,对IL-6表达水平的抑制率为38%。结论天麻素可降低血脑屏障的通透性,其通过减低炎症反应而对局灶性脑缺血损伤发挥保护作用。
Objective To investigate the role of Gastrodine(Gas) in focal cerebral ischemia reperfusion injury and illustrate its possible mechanisms. Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion in rats for 2 h and then reperfusion. Gas( 5,10,20 mg /kg) were intragastrically administered at the same time of reperfusion. The neurological deficit score,infarct volume and the extravasation of EB were determined 24 h after reperfusion. The expression of TNF-α,IL-1β,IL-6 proteins were detected by western blot. Results Gas(10,20 mg /kg)ameliorated neurological dysfunction,reduced infarct volume and decreased the extravasations of EB. Gas(20 mg /kg) decreased the expression of TNF-α,IL-1β,IL-6 in penumbra tissues,and the inhibition rates were 24%,34% and 38%. Conclusion Gastrodine exerts protective effect against acute focal cerebral ischemia reperfusion injury by reducing inflammation in brain tissue and permeability of blood-brain barrier.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第5期40-43,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
天麻素
脑缺血
血脑屏障
炎症
Gastrodine
cerebral ischemia
blood-brain barrier
inflammation
