摘要
目的研究香菇多糖对平阳霉素抑制血管内皮瘤细胞的增殖的影响。方法将EOMA小鼠血管内皮瘤细胞分别单用香菇多糖处理(LTN组)、单用平阳霉素处理(BLE组)、香菇多糖联合平阳霉素处理(L+B组)和未做任何处理(CON组),比较4组细胞处理前后细胞增殖和凋亡的差异。结果CON组和LTN组作用后d1-d 7的吸光度无显著差异,但均显著高于BLE组和L+B组(P<0.05);L+B组作用后d1-d7的吸光度显著低于BLE组(P<0.05)。BLE组和L+B组G0/G1期和凋亡率显著高于CON组和LTN组(P<0.05),S期、G 2/M期和PI显著低于CON组和LTN组(P<0.05);L+B组G0/G1期和凋亡率显著高于BLE组(P<0.05),S期、G 2/M期和PI显著低于BLE组(P<0.05)。结论香菇多糖可显著增强平阳霉素对小鼠血管内皮瘤细胞抑制增殖及促进凋亡的作用。
Objective To study the effects of Lentinan on bleomycin A5’s inhibition effects in hemangioma-derived endothelial cell. Methods Hemangioma-derived endothelial cell line EOMA were divided into LTN group (only lentinan treatment), BLE group (only bleomycin A 5 treatment), L+B group (Lentinan and bleomycin A5 treatment) and CON group (no lentinan and bleomycin A5 treatment). Cell proliferation, cell cycle, PI and apoptosis were detected and compared among four groups. Results The differences of absorbance in LBE group was significantly higher than that in L+B group after treatment for d 1-d 7 (P<0.05), but both of them were significantly lower than in CON group and LTN group (P<0.05). The G 0/G 1 stage and apoptosis rate in L+B group was significantly higher than in BLE group(P<0.05), while it was significantly lower in S stage, G 2/M stage and PI(P<0.05). Conclusion Lentinan could significantly promote bleomycin A5’s inhibition effects on hemangioma-derived endothelial cell.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第2期20-21,共2页
Chinese Journal of Biochemical Pharmaceutics
基金
国家自然科学基金青年科学基金(30901287)
关键词
血管瘤
平阳霉素
香菇多糖
细胞增殖
凋亡
hemangioma
bleomycin A5
lentinan
cell proliferation
apoptosis
