摘要
对海洋多糖类药物—“872”样品,采用乙醇沉淀分级法得到的5个不同分子量级份(Ⅰ～Ⅴ)。以粘度法测定5个级份特性粘数[η];用激光小角光散射仪(LALLS仪)测定了各级份的重均分子量M_w和第二维利系数A_2;标定出“872”样品的Mark Houwink公式。测得872样品各级份/特性粘数。
This paper describes the measurement of molecular weight and the calibration of the constants K and α of sample '872',Five '872' samples with different molecular wights were prepared by alcohol precipitating.The intrinsic viscosities of these samples are measured with a Ubbelohde viscometer and their absolute weight-average molecular weights (Mw)and second virial coefficients were determined with a Low Angle Laser Light Scattering Photometer (LALLS).The constants K and a of the Mark-Howink formula were obtained by plotting or the least square method.The intrinsic viscosities of all five' 872' samples fell in the range of 9.32-10.68 ml/g,the weight-average molecular weights (Mw) were between 11404-13806,and the second Virial coefficients (A2) were between 1.66×10-3~2.88×10-3.The constants K and a were 9.6294×10-3 and 0.7358 respectively.Hence the sample '872' Mark-Houwink formula is:
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
1992年第S1期43-51,共9页
Periodical of Ocean University of China
关键词
海藻多糖
激光小角光散仪
分子量和分布
测定
特性粘数
瑞利因子
sea weed
polysacchride
Low Angle Laser Light Scattering Photometer
molecular weight and distribution intrinsic viscosity
Rayleigh factor
determination
