摘要
目的 :观察壳多糖 (chitosan)保肝的药理作用。方法 :按 2 0 0mg·kg 1 的剂量给予小鼠灌胃给药 ,观察壳多糖对四氯化碳致小鼠急性肝损伤的组织学变化及其对转氨酶、脂质过氧化的影响。结果 :壳多糖组小鼠肝细胞变性、坏死明显轻于造模组 ;壳多糖组ALT水平 ,血浆、肝匀浆过氧化脂质 (LPO)及血浆丙二醛 (MDA)含量亦显著低于造模组 ,组间比较差异具有极显著性或显著性 (P <0 .0 1或P <0 .0 5 )。结论 :壳多糖对小鼠急性实验性肝损伤有明显的保肝降酶作用 ,机制可能与其抗自由基损伤。
Objective:To study the protective effect of chitosan on the liver. Method:Mice of the Kunming strain were given each chitosan (200 mg·kg -1) by gastrogavage for 9 consecutive days. A liver injury model was produced in these mice by injecting 600 mg·kg -1 of carbon tetrachloride (CCl 4)into the peritoneal cavity of each animal on the 3 rd, 6 th and 9 th days of the experiment,respectively. Effects of chitosan on the liver histology, serum aminotransferase and lipid peroxidation (LPO) in the CCl 4 intoxicated mice were kept under observation. Results:In mice treated with chitosan, degenerative and necrotic changes of the liver cells were apparently milder than those of mice untreated. The activity of serum alanine aminotransferase (ALT), the content of plasma and liver homogenate LPO as well as that of plasma malonyldialdehyde (MDA) were significantly lower in mice treated with chitosan. Difference between the two groups of mice were significant (P<0.01 or P<0.05). Conclusion:Chitosan was shown to have a striking protective effect on the liver injured by CCl 4. The mechanism underlying the effect of chitosan may be related to its action to antagonize free radicals and inhibit lipid peroxidation.
出处
《医药导报》
CAS
2004年第7期450-451,共2页
Herald of Medicine
关键词
壳多糖
护肝
四氯化碳
Chitosan
Hepatoprotective effect
Carbon tetrachloride
Experimental study
