摘要
乙型肝炎病毒(hepaitis B Virus,HBV)是最小的DNA病毒之一,不仅它的HBsAg、HBeAg、
Two-target ribozyme against HBV
was constructed and expressed in HHCC and 2. 2.
15. eukaryotic cells The recombinant plasmid
pBBS212-Rz containing two--target ribozyme gene
and plasmid pl. 2 Ⅱ carrying adr-subtype HBV
genome were cotransfected into HHCC, or
pBBS212--Rz was transfected into 2. 2. 15 cells. At
day 5 after cotransfection, HBsAg was detectable
in the supernate of cultured HHCC, and the ratio
of HBsAg to HBeAg was the highest by ELISA
method. HBeAg was found in the cytoplasm of
HHCC at day 7 after cotransfection and reached the
highest level at week two. HBsAg and HBeAg in
cotransfected cells could stabely express for two
months. The expression of ribozyme in
cotransfected HHCC and 2. 2. 15 cells could also be
detectable by in-situ hybridization after selection
by hygromycin B for two week. The level of
HBeAg was reduced by 50%～65% in the
cotransfected HHCC cells, and 42%～48% in the
supernate of pBBS212--Rz--transfected 2. 2. 15 cells,
but HBsAg in both cell lines was not inhibited.
The level of HBcAg in the cytoplasm of
cotransfected HHCC cells was also reduced by 62%
by using immunohistochemical technology, image
analysis system and laser co--focal imaging system,
etc. These results indicated that the two--target
ribozyme could inhibit HBV expression in cells.
出处
《传染病信息》
1998年第3期118-119,共2页
Infectious Disease Information
