摘要
目的：为探讨异种免疫耐受的可能性。方法：以豚鼠－大鼠心脏移植建立超急排斥反应模型，在眼镜蛇毒因子（ＣＶＦ，３０Ｕ／ｋｇ）及环磷酰胺（ＣｙＰ）的覆盖下，将供者特异性或非特异性血管内皮细胞以４．０×１０６个／只，注入受者的门静脉，２周后行颈部心脏移植。结果：移植供心存活时间获得延长至（４６．１±５）ｍｉｎ，加用ＣｙＰ后延长尤为明显（５４．５±９．７ｍｉｎ），同时血管内皮细胞溶解率下降，ＣｙＰ对移植物存活也有一定的延长作用（３４．６±８．７ｍｉｎ），而非特异性血管内皮细胞则无此作用。结论：在豚鼠－大鼠异种移植模型中，供者特异性血管内皮细胞经门静脉径路注射可诱导受者产生一定程度的免疫耐受。这种耐受的产生机制尚需进一步研究。
Objectives: Induction of
allograft and concordant xenograft tolerance has been successful in many animal models.In
this study,guinea pigtorat discordant xenograft tolerance was attempted. Methods:
donorspecific guineapig arterial endothelial cells were isolated and amplified by in vitro
culture,then injected(4.0106/rat) into portal vein after recipient rats were pretreated with Cobra
Venom Factor( CVF, 30 U/kg) alone or in combination with Cyclophosphamide( CyP) .
Guineapig hearts were transplanted heterologically 14 days thereafter. Results: Cardiac
survival time of treated rats was much longer than control(468.5 min vs 16.44.7
min,P<0.05),while the survival time could be prolonged still further by using CyP(54.59.7
min,P<0.05).Nonspecific vascular endothelial cell couldn't prolong graft survival
time,splenectomy and CyP could partially prolong the survival time(34.68.7 min and 35.4 15.5
min,P>0.05 vs control) . A close correlation between vascular endothelial cell lytic ratio and
graft survival time in each group was observed.Histological study found no apparent
difference among all groups qualitatively and quantitatively at the end of rejection.
Conclusions: The results suggested that injection of donorspecific endothelial cells into
recipient rats portal vein could lead to xenograft tolerance of transplanted heart between
gunieapigs and rats, but it couldnt prevent HAR completely. Vascular endothelial cell lytic
ratio was helpful in studying AR in vitro.
出处
《医学研究生学报》
CAS
1999年第3期34-36,共3页
Journal of Medical Postgraduates
基金
国家自然科学基金
关键词
血管内皮细胞
免疫耐受
门静脉
异种移植
Endothelial cells
Xenograft tolerance
Cobra
Venom Factor
Portal vein
