摘要
目的建立定量检测肿瘤患者血浆中hTERT mRNA水平的实时荧光RT-PCR方法,探讨其在急性髓细胞性白血病(AML)和喉鳞状细胞癌的诊断与疗效观察中的应用价值。方法采用TriZOLLS提取21例AML患者、23例喉鳞状细胞癌患者以及25例健康志愿者血浆总RNA,逆转录后利用Taqman技术对hTERT mRNA进行实时定量检测。结果健康志愿者血浆hTERT mRNA水平(NhTERT)为1.2±0.8,喉鳞状细胞癌患者手术前与手术后2天,NhTERT分别为11.8±8.3和8.0±5.7(P=0.03),AML首次治疗前与完全缓解患者分别为13.5±8.5和2.4±2.0(P<0.001)。与健康志愿者比较,AML首治患者与手术前喉鳞状细胞癌患者血浆hTERT mRNA水平显著升高。结论采用实时荧光RT-PCR能够有效地检测血浆中hTERT mRNA水平,为AML和喉鳞状细胞癌患者的诊断与疗效观察提供信息。
Objective To set up a real-time fluorescent RT-PCR and to quantitate the levels of hTERT mRNA in plasma of patients with AML and laryngeal squamous cell carcinoma and to investigate the value for cancer diagnostic and curative effect.Methods 25 healthy volunteers,23 patients with laryngeal squamous cell carcinoma and 21 patients with AML were recruited in this study.Plasma total RNA was extracted from plasma with TriZOLLS and mRNA was transcribed reversely into cDNA,then real time quantification of hTERT mRNA was performed with Taqman technology.Results NhTERT in plasma in healthy volunteers was 1.2±0.8.For patients with laryngeal squamous cell carcinoma,NhTERT was 11.8±8.3 before surgery and 8.0±5.7 two days after surgery(P=0.03).NhTERT in plasma in patients with AML at initial presention and at complete remission were 13.5±8.5 and 2.4±2.0 respectively(P<0.001).Comparing with healthy volunteers,hTERT mRNA in plasma rose significantly in patients with AML at initial presention and laryngeal squamous cell carcinoma before surgery.Conclusion The hTERT mRNA in plasma can be detected and quantified effectively with real-time fluorescent RT-PCR and the detection of hTERT mRNA in plasma can provide useful data for diagnosing and observing curative effect of AML and laryngeal squamous cell carcinoma.
出处
《分子诊断与治疗杂志》
2009年第4期222-226,共5页
Journal of Molecular Diagnostics and Therapy
基金
湖北省科技攻关项目(2002AA304B10)
关键词
急性髓细胞性白血病
喉鳞状细胞癌
实时
端粒酶
逆转录酶
聚合酶链反应
Acute myelogenous leukaemia
Laryngeal squamous cell carcinoma
Real time
Telomerase
Reverse transcriptase
Polymerase chain reaction
