摘要
近年来的研究发现HBV具有泛嗜性。白细胞也是其靶细胞之一。为明确HBV感染外围血白细胞的意义及其与免疫功能的关系。本文采用Southern分子杂交和斑点分子杂交法进行研究。材料及方法一、病例选择:根据1984年南宁会议标准选择乙型肝炎病人61名。47人经肝活检证实诊断,其中急性肝炎8名,慢性肝炎53名。慢性肝炎中的33名与全部急性肝炎患者以斑点分子杂交法检测。另20名慢性肝炎患者以Southern分子杂交法进行研究。二、斑点杂交和Southern分子杂交斑点杂交:将静脉血中分离的白细胞(1×10~5个)充分消化后,点于硝酸纤维素膜上,以后步骤同血清HBV DNA检测。
PBL HBV DNA from 41 hepatitis patients and 4 normal controls were ex-amined by spot blot hybridazition ith ^(32)p labeled HBV DNA probe. HBV DNAwas observed in PBL of 4/8 (50％) acute hepatitis patients, of 19/33 57.6％)chronic patients. There was no close correlation between PBL HBV DNA andpatients of HBV serological arkers. The differences of PBL HBV DNA posi-tive rate in various patterns of HBV serological markers was not bvious (p>0.05). There was also no correlation between PBL HBV DNA and liver dama-ge. It was also found that the evels of α-interferon in PBL HBV DNA positivegroup were higher than in negative group but he ifference was not obvious(p>0.05). Using Southern blot technique to study other 20 CAH atients livertissue and PBL. 3 showed HBV DNA in their liver and none in their PBL.
出处
《临床肝胆病杂志》
CAS
1989年第1期27-28,共2页
Journal of Clinical Hepatology
