摘要
目的研究直肠癌及正常直肠黏膜中微小RNA(miRNA)表达谱差异,寻找并验证具有差异表达的miRNA。方法提取3例直肠癌患者的癌组织及其对应远端正常直肠黏膜中的miRNA,采用微阵列芯片分析,获得直肠癌miRNA表达谱。另取15例直肠癌患者手术切除标本的癌组织及正常黏膜组织,采用实时定量RT-PCR法对其中具有表达差异的hsa-miR-187*和hsa-miR-224进行进一步验证。结果共筛选出70个直肠癌相关的差异表达miRNA,其中33个表达上调,37个表达下调。其中肿瘤/正常倍数小于0.5的有22个,肿瘤/正常倍数大于4的有17个。在另外15对验证样本中证实hsamiR-187*在直肠癌中表达下调(P<0.001),hsa-miR-224表达上调(P<0.001)。结论直肠癌和正常直肠黏膜相比有其特异的miRNA表达谱,miRNA在直肠癌的发生中可能起到重要作用。
Objective To compare the expression profiles of microRNAs(miRNAs) between rectal adenocarcinoma and normal rectal mucosal tissues, further to identify the differentially expressed miRNA possibly associated with the development of rectal cancer. Methods miRNA expression profiles of three rectal adenocarcinoma samples and related normal rectal mucosal tissues were performed by miRNA microarray analysis. Real-time quantitative RT-PCR was performed to confirm the expressions of hsamiR-187* and hsa-miR-224 in another 15 cases of rectal adenocarcinoma. Results A total of 70 miRNAs were found to be differentially expressed in rectal adenocarcinoma and normal tissues, including 33 upregulated miRNAs and 37 down-regulated miRNAs. Compared with normal rectal samples, hsa-miR-187* expression was down-regulated in rectal adenocarcinoma(P<0.001), while hsa-miR-224 expression was upregulated(P<0.001). Conclusion Compared with that in normal rectal mucosal tissues, the specific miRNA expression profile might play an important role in the development of rectal adenocarcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2014年第6期602-605,共4页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金资助项目(81072034)
河北省自然科学研究计划资助项目(C2011206103)
河北省科技计划资助项目(12396105D)
