摘要
本研究旨在探索膜相关转运蛋白(MATP)与羊驼毛色形成的相关性。以白色和棕色的成年羊驼为研究对象,主要用荧光定量PCR技术分析MATP基因在不同毛色羊驼皮肤的相对表达量、免疫组织化学法和Western blot对MATP蛋白在不同毛色羊驼皮肤中的定位和表达进行研究。荧光定量PCR结果显示,棕色羊驼中MATP的mRNA相对表达量是白色羊驼的3.047倍(P<0.05);Western blot结果表明,羊驼皮肤组织总蛋白提取物中存在相对分子质量约53ku的产物,棕色羊驼的皮肤平均蛋白表达量显著高于白色羊驼(P<0.05);免疫组化结果显示,MATP在羊驼皮肤毛囊的表皮、根鞘及毛球部均表达。MATP与黑色素细胞分布及含量关系密切,可能通过调控黑色素生成从而参与羊驼毛色形成过程。
The objective of this study was to explore the relationship between MATP and alpaca coat color formation.Real-time quantitative PCR,western blotting and immunohistochemistry were applied for examining the mRNA and protein expression level of MATP in alpaca skin of different coat colors.The gene expression quantity of MATPin brown alpaca's skin tissue was 3.047times compared with white alpaca.The protein expression of MATP in brown alpaca skins was significantly higher than that in white alpaca skins(P<0.05).The MATP was demonstrated to express in epidermis,outer root sheath and dermal papilla of hair follicular by immunohistochemistry.Our findings suggested that MATP was involved in the process of alpaca coat color formation.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第3期502-508,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家"863"计划(2013AA102506)
农业部"948"项目(2011-Z33)
山西农业大学科技创新基金(201204)
