摘要
目的探讨体外受精(IVF)和精子卵胞浆内注射技术(ICSI)治疗周期中体外培养囊胚形成的影响因素。方法将卵裂期第3d(D3)冷冻后剩余胚胎进行体外延长培养至囊胚期,观察囊胚形成情况。结果共收集7438个冷冻后剩余胚胎,经序贯培养,形成1382个优质囊胚(18.58%)。D3胚胎中6个细胞的优质囊胚形成率(10.34%)显著高于4-5个细胞的优质囊胚形成率(5.45%),差异有统计学意义(P=0.011);7-9个细胞的优质囊胚形成率(19.95%)显著高于6个细胞的优质囊胚形成率(10.34%),≥10个细胞的优质囊胚形成率(27.47%)显著高于7-9个细胞的优质囊胚形成率(19.95%),差异均有统计学意义(P<0.001)。D2胚胎中4个细胞的优质囊胚形成率(25.40%)显著高于3个细胞、5个细胞的优质囊胚形成率(10.26%,15.41%),差异有统计学意义(P<0.001)。碎片评级I-II级D2、D3胚胎的囊胚形成率均显著高于III-IV级的胚胎,差异有统计学意义(P<0.001)。结论体外延长培养能有效筛选具有发育潜力的胚胎,优质囊胚形成与D2、D3胚胎的卵裂球数及碎片评级密切相关。
Objective To explore related factors for blastocyst development during the in-vitro fertilization(IVF) and Intracytoplasmic sperm injection(ICSI) cycles.Methods D3 surplus embryos after embryo t ransfer or Vitrification from IVF/ ICSI cycles were cultured into blastocyst by the sequential method,then the blastocyst development were observed.Results A total of 7438 surplus embryos after embryo transfer or Vitrification were collected and formed 1382 high-quality blastulas(18.58%) af ter the sequential culture.At D3 cleavage stage,6-cell embryo had the significantly higher rate of high-quality blastula formation(10.34%) than 4 or 5-cell embryo(5.45%)(P=0.011);7-9cells embryo had the significantly higher rate of high-quality blastula formation(19.95%)than 6-cell embryo,≥10-cell embryo had the significantly higher rate of high-quality blastula formation(27.47%)than 7~ 9-cell embryo(P<0.001).At D2 cleavage stage,4-cell embryo had the significantly higher rate of high-quality blastula formation(25.40%)than 3 or 5-cell embryo(10.26%,15.41%)(P<0.001).At D2/ D3 cleavage stage,the embryo with I-IIgrade fragment had the significantly higher rate of high-quality blastula formation than the embryo with III-IV grade fragment(P<0.001).Conclusion In-vitro blastocyst culture can have significant advantage to screen the high-quality embryo with development potential.The blastocyst formation has close relationship with D2/D3 embryo blastomere number and fragment.
出处
《江西医药》
CAS
2013年第4期305-308,共4页
Jiangxi Medical Journal
关键词
体外受精
囊胚培养
卵裂球
胚胎碎片
In-vitro fertilization
Blastocyst culture
Blastomere
Embryo fragment
