摘要
目的:分析土壤缺水对黄芩SOD,APX,DHAR,MDHAR基因表达的影响。方法:利用受控实验对黄芩进行土壤缺水胁迫;利用半定量RT-PCR对基因转录水平进行分析。结果:与对照组相比,胁迫70 d后,APX基因转录水平显著降低;胁迫30,50 d后,DHAR基因转录水平显著降低;胁迫50 d后,MDHAR1基因有显著降低。结论:在土壤缺水的条件下,维生素C(AsA)作为一种重要的抗氧化剂在过氧化氢清除系统中可能发挥主要作用,其与黄芩素积累可能处于竞争的关系。
Objective:To analysis the effects of water deficit on the transcript level of SOD,APX,DHAR and MDHAR genes in Scutellaria baicalensis.Method:Three-month-old S.baicalensis was in glasshouse under water deficit stress,and the transcript level of SOD,APX,DHAR and MDHAR genes were analysis utilized semi-quantitative RT-PCR.Result:Compared with the control group,a significant decline of the transcriptional level of APX gene was observed at 70 days after water deficit.The transcript level of DHAR gene was reduced at 30 and 50 days after water deficit.And MDHAR1 gene was significant declined at 50 days.Conclusion:AsA which is an important antioxidant plays a major role in hydrogen peroxide clear system under water deficit,and maybe have an antagonistic effect to the accumulation of baicalein.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第2期186-188,共3页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09301-005-03)
北京市科技新星项目(2008B82)
中国博士后科学基金特别资助项目(201003228)
