摘要
目的 观察TRAIL对前列腺癌细胞PC 3M的生长抑制作用。方法 采用MTT法检测不同浓度TRAIL对人非激素依赖性前列腺癌细胞株PC 3M的生长抑制率 ,原位末端酶标记技术检测细胞凋亡。免疫组化法观察bcl 2的表达。结果 TRAIL可有效地抑制PC 3M细胞生长 ,具有时间、浓度依赖性特点。经药物作用后 ,前列腺癌细胞凋亡明显增多 ,凋亡率随作用时间的延长而增高 ,PC 3M细胞中的bcl 2基因表达无显著变化。结论 TRAIL蛋白可显著抑制前列腺癌细胞PC 3M生长 ,其诱导细胞凋亡不依赖bcl
Objective To observe the effect of TRAIL protein on the growth of human prostate cancer cells and illustrate its mechanisms.Methods The growth inhibition rates of human prostate cancer cell line PC 3M by various concentration TRAIL protein were studied by MTT method,cell apoptosis of PC 3M cells was observed by SP immunohistochemical method.Results TRAIL could inhibit the growth of PC 3M effectively with the increase of apoptosis cell, which depended on the action time and concentration of drug.bcl 2 expression of PC 3M cell was not changed significantly.Conclusion TRAIL could significantly inhibit the growth of bladder cancer cells.TRAIL induced cell apoptosis did not dependent on bcl 2 gene.
出处
《肿瘤防治研究》
CAS
CSCD
2004年第7期395-397,F002,共4页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金资助项目 (30 0 70 75 6 )
