摘要
[目的]建立赤芍颗粒饮片中芍药苷的含量测定方法。[方法]采用高效液相色谱法,以symmetry C18(46mm×150mm,5μm)为色谱柱,以乙腈∶水(15∶85)为流动相,检测波长230nm;温度:30℃。[结果]芍药苷浓度在10～400μg/ml范围内与色谱峰面积有良好的线性关系,回归方程:y=21741x-365,r=0.9999(n=6),平均加样回收率为99.77%,RSD为1.58%(n=6)。[结论]该方法简便、灵敏、结果准确,可用于芍药苷含量的测定。
[Objetive]To determine the contents of paeonitlorin in prepared granul of paeonia lactiflon,the HPLC method was established.[Method]Symmetry C18(46mm×150mm,5μm)was used;the mobile phases consisted of acetonitrile—water(15∶85),detecting wave length was at 230nm;and the temperature was 30 degree.[Result]Paeonitlorin showed a good linear correlation within the concentration of 10-400μg/ml,the regress equation was y=21741x-365,r=0.9999(n=6),the average recovery was 99.77%,RSD=1.58%.[Conclusion]This method is simple,sensitive,accurate and suitable for the determination the contents of paeonitlorin.
出处
《浙江中医药大学学报》
CAS
2011年第1期86-87,共2页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药科学研究基金计划(No:2010ZA083)~~
