摘要
目的观察亚砷酸(As_2O_3)对人肺腺癌的诱导凋亡作用及对LBP基因表达的影响及可能机制。方法选用人肺腺癌A549细胞系,应用体外细胞培养法,流式细胞术检测As_2O_3对人肺腺癌的诱导凋亡作用;用逆转录-聚合酶链反应(RT-PCR)方法检测LRP mRNA的表达。结果不同浓度的As_2O_3均可诱导A549细胞凋亡。1.0μmol/L、2.0μmol/L的As_2O_3可下调LRP mRNA的表达。结论As_2O_3诱导肿瘤细胞凋亡作用主要是通过下调LRP mRNA表达有密切关系。
Objective To study the apoptosis and mechanism of arsenicals on human lung adenocarcinoma cells. Methods FCM and RT-PCR tests were used to investigate of apoptosis levels induced by arsenic trioxide and the effect on LRP mRNA expression. Results 1.0μmol/L and 2.0μmol/L arsenic trioxide can decrease LRP mRNA expression. Con. clusion Low concentrations may improve apoptosis of lung cancer by decreasing LRP mR- NA expression. As_2O_3 may be used to treat lung cancer but the results are no better than cis- plation.
出处
《中国老年保健医学》
2004年第4期32-34,共3页
Chinese Journal of Geriatric Care
