摘要
目的探讨XRCC5 C74468A和G74582A单核苷酸多态性(single nucleotide polymorphisms,SNPs)与中国河北人群肺癌遗传易感性的关系,及其对肺癌TNM分期及淋巴结转移的影响。方法应用引物引入限制性内切酶分析-聚合酶链反应(primer-introduced restriction analysis-polymerase chain reaction,PIRA-PCR)和聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法检测352例肺癌患者和404例健康对照者XRCC5 C74468A和G74582A的基因型,并以非条件Logistic回归模型分析基因多态性与肺癌发病风险的关系,及其对肺癌TNM分期和淋巴结转移的影响。单体型分析采用EH软件。结果吸烟可显著增加中国河北人群肺癌的发病风险,经性别、年龄校正后的相对风险度的比值(odds ratio,OR)为3.44[95%可信区间(confidence interval,CI)=2.37～4.98]。XRCC5基因C74468A和G74582A多态等位基因和基因型分布在肺癌组与健康对照组间差异无统计学意义(P>0.05);XRCC5 C74468A多态基因型分布在Ⅱ期肺癌患者组与健康对照组间差异有统计学意义(x^2=9.831,P<0.01)。与C/C基因型相比,携带A等位基因(A/C+A/A基因型)可显著降低Ⅱ期肺癌的发病风险,经性别、年龄和吸烟校正后的OR值为0.46(95% CI=0.28～0.77)。两个多态位点联合分析显示,单体型分布在肺癌组与健康对照组间差异无统计学意义(P>0.05)。结论 XRCC5 G74582A多态可能与中国河北人群肺癌的发病风险无关。而XRCC5、C74468A多态A等位基因(A/C+A/A)可能降低河北人群Ⅱ期肺癌的发病风险。
Objective This study was to investigate the correlations of XRCC5 C74468A and G74582A SNPs with genetic susceptibility,TNM staging and lymph nodes metastasis of lung cancer in a population of Hebei province of China.Methods XRCC5 SNPs(C74468A and G74582A)were genotyped by primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR)and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 352 lung cancer patients and 404 healthy controls.Unconditional logistic regression was used to analyze the XRCC5 C74468A and G74582A SNPs with the risk of lung cancer. Results Smoking significantly increased the risk of lung cancer in a population of Hebei Province of China [age and gender adjusted odds ratio(OR)=3.44,95% confidence interval(CI)=2.37～ 4.98].The allelotype and overall genotype distribution of the XRCC5 C74468A and G74582A SNPs among the lung cancer patients and healthy controls were similar(P>0.05).However, when stratified by clinical staging,frequency of the XRCC5 C74468A genotype distributions were significantly different between the lung cancer patients in stage Ⅱ and healthy controls(χ~2= 9.831,P<0.01).Compared with the C/C genotype,A allele(A/C+A/A genotype) significantly reduced the risk of developing lung cancer in stage Ⅱ(age,gender and smoking status adjusted OR=0.46,95%CI=0.28～0.77).Combined analysis of the XRCC5 SNPs showed that the haplotype distribution in lung cancer patients was not significantly different from that in healthy controls(P>0.05).Conclusion XRCC5 G74582A SNP may have no relation with susceptibility to lung cancer in a population of Hebei province of China.However,A allele(A/C +A/A genotype)of XRCC5 C74468A may significantly reduce the risk of developing lung cancer in stage Ⅱ.
出处
《河北医科大学学报》
CAS
2009年第10期1012-1016,共5页
Journal of Hebei Medical University
基金
河北省普通高等学校强势特色学科肿瘤学建设经费资助项目
关键词
肺肿瘤
多态性
单核苷酸
疾病遗传易感性
lung neoplasms
polymorphism,single nucleotide
genetic predisposition to disease
