摘要
目的:体外检测骨髓基质细胞(bone marrow stromal cells,BMSCs)的条件培养基对脊髓运动神经元的影响。方法:体外培养SD大鼠的BMSCs,并收集其条件培养基。实验组脊髓运动神经元用条件培养基培养,对照组用NB培养液。观察两组运动神经元形态学指标,测量细胞突起长度。MTT法检测两组细胞活力。结果:实验组运动神经元的细胞活力、突起长度与对照组相比差异均有统计学意义(P<0.01)。结论:BMSCs合成和分泌神经营养活性物质对脊髓运动神经元有维持存活和促进突起生长的作用。
Objective:To investigate the effects of bone marrow stromal cells(BMSCs) secretory factors on cultured spinal cord motor neuron.Methods:BMSCs were cultured in vitro.The spinal cord motor neurons in experimental group were grown in conditioned medium which was collected from BMSCs cultures(every 72 h);the cells in control group used NB correspondly.Morphological changes of motor neuron were observed under light microscope.Activity of motor neuron was detected with MTT method.Results:Compared with the control...
出处
《南通大学学报（医学版）》
2009年第6期415-416,419,共3页
Journal of Nantong University(Medical sciences)
基金
国家自然科学基金资助项目(3087643)
江苏省高校自然科学基础研究面上项目(08KJD310015)
