摘要
研究了不同有机碳源对转hTNF α基因聚球藻70 0 2细胞的生长及光合作用的影响。通过对混合营养培养初时及生长 2d后细胞光合放氧的比较 ,发现经过一个对有机碳利用的适应和诱导过程后 ,适宜的有机碳源可以显著促进细胞的光合作用及生长 ;同时还发现 ,有机碳源的加入抑制了低CO2 浓度下培养的藻细胞的初始光合放氧 ;然而 ,当加入碳酸氢钠 1g/L后 。
The effects of organic carbon sources on photosynethesis and growth of trans hTNF α Synechococcus sp. PCC7002 were studied. Several organic carbon compounds, such as sucrose, glucose, glucosamine were investigated. The net photosynthetic rates of cells during the beginning and exponential phases of mixotrophic growth were investigated and compared. It was shown that organic carbon sources accelerated cell growth markedly after inducing or activating the sugar uptake system (Fig.1). After adaptation to the presence of sugar for 2 d, the oxygen evolution rate of mixotrophic culture was double that of photoautotrophic culture (Fig.3). Cells adapted to low CO 2 showed a reduced photosynthetic O 2 evolution at zero time after sugar addition when compared to photoautotrophic cells.This decline was correlated with a decrease in carbonic anhydrase activity (Fig.2). But when NaHCO 3 1 g/L was added, the net photosynthetic rate of cells was noticeably enhanced by organic carbon sources (Fig.4), which indicated that there was some relation between that of organic carbon and that of inorganic carbon.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2003年第5期405-408,共4页
Journal Of Plant Physiology and Molecular Biology
关键词
转基因聚球藻
光合作用
有机碳源
混合培养
transgenic cyanobacteria
photosynthesis
organic carbon sources
mixotrophic Synechococcus
