摘要
目的研究龟板、黄芪、丹参、党参在体外对K562细胞Gγ-珠蛋白mRNA表达的上调作用。方法以K562细胞为体外模型,应用荧光定量RT-PCR技术研究药物诱导后Gγ-珠蛋白基因mRNA表达水平的变化。结果龟板、黄芪、丹参、党参作用K562细胞5 d后,Gγ-珠蛋白mRNA的表达增加(最高达3.7倍)。与丁酸钠组比较,黄芪组诱导K562细胞Gγ-珠蛋白基因mRNA的表达可持续3~5 d,而丁酸钠组仅1 d。结论龟板、黄芪、丹参、党参有望成为一种有效的γ蛋白基因诱导剂。
OBJECTIVE To study the effects of accumulation of γ-globin mRNA on K562 cells induced by Tortois plastron,Radix Astragali,Radix Astragalus membranaceus and Radix Codonopsitis pilosula in vitro.METHODS K562 cells were cultured for 5 days with different mediums of Tortois plastron,Radix Astragali,Radix Astragalus membranaceus and Radix Codonopsitis pilosula.γ-globin mRNA was quantitated by RT-PCR.RESULTS The expression of Gγ-mRNA increased significantly(maxmum to 3.7 fold) in K562 cells after the treatment of...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第3期260-262,共3页
West China Journal of Pharmaceutical Sciences
关键词
龟板、黄芪、丹参、党参
K562细胞
Gγ-mRNA
Tortois plastron
Radix Astragali
Astragalus membranaceus
Codonopsitis pilosula
K562 cells
Gγ-mRNA
