摘要
目的证明正常人与癌症患者血清拉曼光谱中频移为1006、1168和1523 cm^(-1)处的拉曼峰是由人体血清中β-胡萝卜素分子产生的；从能量角度,分子水平探讨细胞癌变的原因。方法应用拉曼光谱技术检测β-胡萝卜素溶液样品。将β-胡萝卜素样品溶解在浓度为95%的CCl_4分析纯溶剂中,配制成浓度为0．01mg/ml(浓度大于人体血清中β-胡萝卜素的正常浓度值)的溶液,选择氩离子激光器输出波长为488．0nm谱线作为激发光源,照射β-胡萝卜素溶液样品,经弱信号检测系统处理后,记录输出信号。结果β-胡萝卜素溶液样品的3个拉曼频移值分别为1003、1164和1524 cm^(-1)；并发现β-胡萝卜素样品的拉曼峰随着照射时间的增加,其强度下降很快,发生了明显的光分解现象,而人体血清中β-胡萝卜素的拉曼峰随着照射时间的增加,其强度不下降,没有观察到光分解现象。结论正常人与癌症患者血清拉曼光谱是由人体血清中β-胡萝卜素分子产生的；血清中可能存在着一种传能机制,且正常人和癌症患者血清中这种传能机制是不同的。
Objective To prove that the frequency shifts 1 006,1 168 and 1 523 cm^(-1)of the Raman spectrum of human serum are caused byβ- carotene in human serum and to investigate reasons of cancer on molecule level from the energy viewpoint. Methods Raman spectrum ofβ-carotene solution was measured.β-Carotene sample was dissolved in the solvent of 95% CCl_4.The concentration of the sample was 0.01%.Theβ-carotene solution was excitated by 488.0 nm laser light.The signal from detective sys- tem of weak signal was recorded. Results The frequency shifts of the Raman spectrum ofβ-carotene sample were 1 003,1 164 and 1 524 cm^(-1).It is discovered that the intensity of Raman spectrum ofβ-carotene sample was decreasing with increasing time.Theβ-carotene sample was decomposed by 488.0 nm laser light.But the intensity of Raman spectrum ofβ-carotene in human serum was not decreased with increasing time.We had not discovered the decomposed phenomena ofβ-carotene in human serum. Conclusions The frequency shifts 1 006,1 168 and 1 523 cm^(-1) of the Raman spectrum of human serum are caused byβ-carotene in human serum.It is possible that there is a mechanism of energy transfer in human serum.The mechanism of energy transfer is not same in serum of normal human and cancer patient.
出处
《中国激光医学杂志》
CAS
CSCD
2006年第5期283-285,共3页
Chinese Journal of Laser Medicine & Surgery
