摘要
目的探讨淮南地区幽门螺杆菌(Helimbacter pylori,Hp)对克拉霉素耐药情况及其耐药分子机制。方法用E-test和琼脂稀释法进行克拉霉素药敏试验,提取Hp基因组DNA,PCR扩增Hp 23S rRNA基因,并用PCR-限制性片段长度多态性(RFLP)分析克拉霉素耐药机制。结果淮南地区克拉霉素耐药率为9.15%(27/141),克拉霉素耐药与性别、年龄无关。PCR从Hp基因组DNA中扩增出425bp的Hp 23S rRNA基因,PCR-RFLP检测,27株耐药菌株均可被Bbsl酶切成332、93bp两个片段,未检测到被BsaⅠ酶切的耐药菌株。结论淮南地区克拉霉素耐药率较高,耐药菌株存在23S rRNA基因功能区V2143位点A-G突变。
To understand the resistance of Helicobacter pylori to clarithromycin in Huainan area and to explore the molecular mechanism for drug-resistance,the susceptibility test of H pylori to clarithromycin was performed by means of E-test and the agar dilution method of assay. Meanwhile,the 23S rRNA V-domain gene of H pylori was amplified by PCR and the molecular mechanism for clarithromycin-resistance was analyzed by PCR-restriction fragment length polymorphism(RFLP). The results showed that the rate of clarithro...
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2008年第3期243-246,共4页
Chinese Journal of Zoonoses
基金
安徽省教育厅自然科学研究项目(No2006kj120C)
安徽省淮南市科技计划项目
关键词
幽门螺杆菌
克拉霉素耐药
分子机制
淮南地区
Helicobacter pylori
clarithromycin resistance
molecular mechanism
Huainan
