摘要
盐酸克伦特罗酶联免疫反应测试盒适用于尿样、组织和饲料等样品中克伦特罗残留的定量检测。克伦特罗作为一种主要的β-兴奋剂应用于畜禽肉制品生产中,用以提高肌肉和脂肪的比例并加快生长速度。由于克伦特罗可作为肌肉舒缓剂用于人类疾病治疗,在畜禽中过量残留可能会对消费者造成危害,为此,被禁止在食品生产中应用。根据美国FDA和WHO规定,在肌肉和脂肪中克伦特罗的含量不能超过0.2ng/g,在肝脏和肾脏中其含量不能超过0.6ng/g。与传统的HPLC或GC-MS方法相比,酶联免疫方法具有操作简便、灵敏度高、成本低的优点。盐酸克伦特罗酶朕免疫反应测试盒为养殖者和政府监督管理部门提供了更准确快速检测克伦特罗残留的方法,该试剂盒的特点包括4方面,第一,该试剂盒的提取方法能达到75%～95%以上的回收率,可以不需用有机溶剂并在10～30min之内完成提取过程。第二,高灵敏度(0.05ng/g);低检测下限(肉类/脂肪0.025ng/g,尿液0.05ng/ml,饲料0.2μg/kg)。第三,检测过程只需不到2h。第四,高重复性。
Clenbuterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol and other related β-agonists in animal tissue,meat,animal feed and urine.Clenbuterol is one of major β-agonists illegally used in livestock production.It can improve the meat/fat ratio in fattened animals and accelerate livestock growth.It also has a relaxing effect on non-striated musculature and can be used as an antiasthmatic and a tocolytic agent in human beings.Therefore,excess clenbuterol residues in livestock products may lead to health risks for consumers.This has led to a prohibition of clenbuterol use in food production.According to the US FDA and WHO,a maximum residue limit for clenbuterol is 0.2 ng/g in muscle and fat,and 0.6 ng/g in liver and kidney.The amount of clenbuterol residues can be determined by methods such as HPLC or GC-MS.However,these traditional methods usually require very expensive preparation procedures.Alternatively,enzyme immunoassay(ELISA) can be used as a screening system,which is simple,rapid,sensitive and cost-effective compared with traditional methods.The unique features of the kit are:a.Rapid(10～30 minutes),and organic reagent-free extraction method for various samples with high recovery(75 %～95 %).b.High sensitivity(0.05ng/g) and low detection limit(0.025 ng/g for meat/tissue,0.05 ng/ml for urine and 0.2 μg/kg for animal feed).c.A quick ELISA assay(less than 2 hours regardless of number of samples.) d.High reproducibility.
