摘要
背景与目的血管内皮生长因子(VEGF)是一种特异地作用于血管内皮细胞的生长因子,它能促进血管内皮细胞增殖及新生血管的生成。本研究的目的是探讨脂质体介导的VEGF反义硫代寡核苷酸(ASODN)对培养的Lewis肺癌细胞合成VEGF的影响。方法将经脂质体包裹并经部分硫代修饰后的VEGFASODN、正义寡核苷酸(SODN)加入培养的Lewis肺癌细胞中,采用RT-PCR技术和免疫组织化学SP法检测肺癌细胞中VEGFmRNA和VEGF蛋白表达,同时测定各上清液刺激下血管内皮细胞生长的受抑情况。结果VEGFASODN能明显抑制Lewis肺癌细胞VEGFmRNA和VEGF蛋白表达,而且可以显著抑制内皮细胞的生长。结论VEGFASODN能抑制肺癌细胞表达VEGF,可以成为肺癌基因治疗的一种新的途径。
Background and objective Vascular endothelial growth factor (VEGF) can specially target the vascular endothelial cells so as to enhance their proliferation and angiogenesis. The aim of this study is to investigate the inhibition effects of VEGF antisense oligodeoxynucleotides (VEGF ASODN) on VEGF expression of Lewis lung carcinoma cells. Methods The phosphorothioate-modified and liposome-encapsulated VEGF ASODN, VEGF SODN were added into the medium of Lewis lung carcinoma cells respectively. VEGF mRNA and protein were detected by RT-PCR and immunohistochemistry. And the proliferation of vascular endothelial cells treated with supernatants was determined. Results VEGF ASODN could significantly inhibit the expression of VEGF mRNA and protein expression in Lewis lung carcinoma cells, as well as the proliferation of endothelial cells. Conclusion It is hopeful that VEGF ASODN may become a new antiangiogenesis therapy strategy in lung cancer.
出处
《中国肺癌杂志》
CAS
2007年第4期275-278,共4页
Chinese Journal of Lung Cancer
基金
黑龙江省科技攻关项目(GDDC190402)资助~~
