摘要
目的:研究尿多酸肽(CDA-2)作用于转移性肺肿瘤小鼠的机制。方法通过注射鼠Lewis 肺癌细胞到C57BL/6小鼠体内建立小鼠转移性肺癌模型。采用HE染色对小鼠肺部肿瘤数、肿瘤大小进行评价。利用Kaplan-Meier (K-M)生存率曲线分析小鼠生存情况。免疫组化方法检测小鼠肿瘤增殖抗原 Ki-67的表达;TUNEL凋亡免疫组化染色观察肿瘤细胞的凋亡。结果经CDA-2治疗后,小鼠的肺肿瘤数和最大肿瘤尺寸均显著低于 PBS组肿瘤小鼠,并且CDA-2呈剂量依赖性抑制肿瘤的生长(均P<0.05)。K-M结果显示,使用PBS组肿瘤小鼠的生存时间显著低于CDA-2治疗组,且随着CDA-2剂量增加肿瘤小鼠生存时间不断延长。免疫组化染色结果显示,肺肿瘤小鼠经CDA-2治疗后,Ki-67阳性肿瘤细胞数显著低于PBS治疗的肿瘤小鼠(P<0.05);而肿瘤凋亡细胞数却明显高于 PBS组(P<0.001)。结论 CDA-2可抑制小鼠肺癌的生长;延长肺癌小鼠的生存期;抑制肿瘤细胞增殖及诱导肿瘤细胞凋亡。
Objective The aim of the study was to investigate the ef ect of CDA-2 on lung tumor in mice tumor models.Methods C57BL/6 mice were used to establish a lung cancer model by intravenous injection of LLC cells.Tumorigenesis was evaluated by tumor multiplicity and maximal tumor sizes.Kaplan Meier(K-M)survival curve was used to analyze survival rate of mice.The expression of Ki-67 in the tumor tissue was measured by immunohistochemical analysis.The apoptosis of tumor cells was analyzed by TUNEL staining.Results Lung tumor mice treated with CDA-2 significantly decreased lung tumor multiplicity and maximal tumor sizes compared to PBS group and depended on CDA-2 does inhibition(P<0.05).K-M survival analysis showed CDA-2-treated tumor mice significant prolonged the life time compared to PBS-treated mice.The numbers of Ki-67-positive tumor cells were significant lower in CDA-2-treated tumor mice than PBS-treated mice(P<0.05);In contrast,lung tumor cells showed significant rate of apoptosis and death when CDA-2 treatment(P<0.001).Conclusions CDA-2 inhibited lung tumor growth in mice;prolonging the life span of lung tumor mice;inhibiting tumor cells proliferation and inducing tumor cells apoptosis.
作者
姚懿雯
吴军录
权文强
万海英
李冬
YAO Yi-wen;WU Jun-lu;QUAN Wen-qiang;WAN Hai-yin;LI Dong(University of Tongji Affiliated Tongji Hospital Department of Clinical Laboratory,Shanghai 200065,China)
关键词
尿多酸肽
肺癌
肿瘤生长
生存期
增殖
凋亡
CDA-2
Lung cancer
Tumor growth
Life span
Proliferation
Apoptosis
