摘要
目的探讨肺癌微粒体环氧化物水解酶(mEH)基因多态性与环境污染因素的交互作用对肺癌发生的形响.方法采用PCR扩增、凝胶电泳对222例肺癌病例和222例健康对照进行基因型分析,非条件logistic回归模型估计比值比并计算交互作用系数.结果病例组和对照组mEH-exon3突变型和杂合型阳性率差异有统计学意义(X^(2)=7.046,P=0.030),而两组间mEH-exort4非野生型阳性率差异无统计学意义(X^(2)=2.674,P=0.263),mEH-exon3突变型增加肺癌危险性(OR=1.99;95%CI:1.21~3.25).在调整混杂因素后,mEH-exon3和exon4非野生型基因与冬季煤炉火墙取暖在肺癌发生中存在明显交互作用,交互作用系数随煤炉火墙取暖使用年限和终生用煤量增加而增加;非野生型exon3和exon4与暴露煤烟或油烟的交互作用均属超相乘模型,交互作用系数分别为2.75和7.34;而与做饭时经常有眼睛或咽喉有刺激感不存在交互作用.结论非野生型mEH基因与室内空气污染因素之间的交互作用在肺癌发生中起重要作用.
Objective To determine the interaction between indoor air pollution and mEH gene polymorphisms.Methods Blood samples from 222 non small cell lung cancer patients and 222 healthy people were characterized by PCR and PCR-RFLP methods.The interaction coefficients were determined through unconditional logistic regression model.Results Significant diferences in the positive rate of mEH-exon3 mutant and the heterozygote were found between case and control groups(x=7.046,P=0.030).But no signifcant difference was found in mEH-exon4 non-wild-type between groups(x^(2)=2.674,P=0.263).mEH-exon3 mutant(OR=1.99;95%CI=1.21,3.25)could significantly increase the risk of lung cancer.After adjusted by confounding variables,significant interactions were found between the use of coal-wall stove and the non-wild type mEH gene.The interaction coffcients were increased with the duration of exposure and quantity of coal consumed.The super multiplication models were established between non-wild type mEH gene and the exposure to soot or oil fume during cooking.The interaction coffcients were 2.75 and 7.34 respectively for exon3 and exon4.No interaction was found between non-wild type mEH gene and irritation of eye or throat during cooking.Conclusion Through the molecular epidemiological techniques,we confrmned indoor air pollution that caused by coal buming was a noticeable lung cancer risk factor.The interaction between the polymorphisms of mEH gene and the indoor air pollution plays an important role in the carcinogenesis of lung.
作者
孙喜文
马玉彦
王波
SUN Xi-wen;MA Yu-yan;WANG Bo(Cancer Research Institute of Harbin Medical University,Harbin 150081,China)
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2007年第S01期30-34,共5页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助项目(30371244)
