摘要
目的探讨糖尿病小鼠模型肝组织氧化损伤.方法雄性昆明种小鼠喂养高脂饮食1周后根据体重随机分成2组,其中一组经小剂量链脲佐菌素注射后诱导成糖尿病小鼠.正常对照组和糖尿病组小鼠各10只,继续喂养高脂饮食,6周后测量2组小鼠末期肝组织中丙二醛、谷胱甘肽、超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、一氧化氮、一氧化氮合酶、肝脏脏器系数及组织形态学观察.结果试验结束时,糖尿病组小鼠一般情况较差,糖尿病小鼠体重显著低于正常组,有统计学意义(t=5.370,P<0.01);肝脏脏器系数显著高于正常组,有统计学意义(t=-6.246,P<0.01);糖尿病组小鼠肝脏组织中丙二醛、谷胱甘肽过氧化物酶、一氧化氮显著高于正常组,有统计学意义(t值分别为:-2.728、-2.660、-4.924,P<0.05或P<0.01),谷胱甘肽、过氧化氢酶、一氧化氮合酶显著低于正常组,有统计学意义(t值分别为:10.639、5.235、3.089,P<0.01),超氧化物歧化酶(t=-0.405,P>0.05)与正常组的差异无统计学意义.光学显微镜下糖尿病组肝脏表现:部分细胞水肿、空泡样变,脂肪滴增大融合.结论本研究表明糖尿病小鼠模型的肝脏存在氧化损伤.
Objective To observe the liver oxidant damage for diabetic model in mice.Methods Male kunming mice were feed with high fat dietary for a week and then were randomly divided into two groups by weight,with 10 mice in each group.One group was induced by small dose streptozotocin(STZ)and obtained STZ-induced diabetic mice,and the other group was regarded as the control.Both of the two groups were feed with high fat dietary.After 6 weeks,the activities of enzymes,such as superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-PX)and nitric oxide synthase(NOS)were measured.Glutathione(GSH),maleic dialdehyde(MDA),nitric oxide(NO)levels in the liver and the liver viscera qutiety were also measured.Liver histological manifestations were observed.Results In diabetes group,there was a significant decrease in body weight,and the activities of GSH,CAT,and NOS decreased significantly(t value were 5.370,10.639,5.235,3.089,espetively,P<0.01).While,the liver viscera quotiety,the levels of MDA,GSH-PX and NO increased remarkably(t value were-6.246,-2.728,-2.660,-4.924,respectively,P<0.01 or P<0.05).The significant difference Was not observed in SOD between the two groups(t value was-0.405,P>0.05).The liver histological damages were observed in diabetes group,light microscope observation showed hepatocytes swelling,ballooned changing and fatty droplets clustering.Conclusion The oxidant damage might exist in the liver diabetic model in mice.
作者
焦士蓉
王波
黄承钰
于爽
阴文娅
JIAO Shi-rong;WANG Bo;HUANG Cheng-yu;YU Shuang;YIN Wen-ya(School of Public Health,Sichuan University,Chengdu 610041,China)
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2007年第S01期115-118,共4页
Chinese Journal of Preventive Medicine
关键词
糖尿病
实验性
小鼠
氧化
损伤
肝
Diabetes mellitus,experimental
Mice
Oxidants
Damage
Liver
