摘要
本实验用BUdR替代胸腺嘧啶核苷的体内实验方法检验棉酚对小鼠精原细胞姐妹染色单体交换(SCE)频率的影响。雄性昆明小鼠连续口服棉酚(4毫克/公斤/日)2周,随之一次腹腔注射吸附于活性炭上的BUdR。经过54小时后,取精原细胞制备中期染色体进行观察。实验结果表明,服棉酚组小鼠精原细胞染色体的畸变率和SCE频率均与对照组无明显差别。而作为阳性对照的致突剂丝裂霉素C(Mitomycin C)所诱致的SCE则明显增高。因而从生殖细胞SCE达一敏感指标提供了证据支持已有报道中认为棉酚不是致突变剂的观点。对所应用以分析精原细胞SCE的体内方法及其在检查避孕药物遗传效应上的意义予以了讨论。
Substitution of thymidine with 5-Bromo-deoxyuridine(BUdR)was performed in vivo in mice for the detection of gossypol effects on the chromosome aberration and sister chromatid exchange(SCE)in spermatogonia.The male Kun-ming mice received a daily dose of 4mg/kg of gossypol for two weeks,followed by a single injec-tion intraperitoneally of BUdR adsorbed on activated charcoal.Metaphases from spermatogonia were prepared for observation 54 hours after the injection.The results demonstrated that the mean frequencies of chromosome aberration and SCE in spermatogonia of gossypol treated mice did not differ significantly from that of the controls.Whereas,the incidences of SCE in mice had been exposed to known mutagen mitomycin C which used as positive controls were markedly increased.This data provide evidence of more sensitive indices for chromosome damage to support the notion that gossypol was not a mutagenic agent.The method of in vivo analysis of SCE in spermatogonia and its application and significance in the detection of genetic damage of contraceptive agents was discussed.
作者
杨育州
薛社普(指导)
Yang Yuzhou;Shieh Sher-Pu(Institute of Basic Medical Research,Academia Medica Sinica)
出处
《解剖学报》
CAS
1982年第2期215-220,235,共7页
Acta Anatomica Sinica
