摘要
目的探讨C57BL/6胎鼠大脑皮质神经元的原代培养方法,并对其纯度进行鉴定。方法用显微解剖方法获取胎鼠大脑皮质组织,通过Papain消化、Hibernate E洗涤、加入含2%B27的无血清的Neurobasal培养液等方法,在体外获得纯化的大脑皮质神经元,并采用NSE免疫荧光染色法检测所培养的神经元细胞的纯度。结果获得的体外培养的皮质神经元细胞生长良好,纯度可达到90%以上。结论本方法利用无血清培养可以获得神经科学所需的高稳定性高纯化的大脑皮质神经元细胞。
Objective To investigate the primary culture method of cortical neurons using the embryonic D15-18 of C57BL/6 mice and to identify the purity.Method The cortical tissues of fetal mice were obtained by microdissection.Purified cortical neurons were obtained in vitro by Papain digestion,Hibernate E washing,which was cultured with serumfree Neurobasal medium containing 2%B27.the purity of cultured neurons was detected by NSE immunofluorescence staining.Results The cultured cortical neurons grew well.Their purity was over 90%.Conclusion Serum-free culture method can be used to obtain highly stable and purified cortical neurons for neuroscience.
作者
何伟亮
田小超
崔丽丽
张祥建
He Weiliang;Tian Xiaochao;Cui Lili;Zhang Xiangjian(Department of Neurology,the Hebei General Hospital,Shijiazhuang 050057,China)
出处
《脑与神经疾病杂志》
2020年第7期407-409,共3页
Journal of Brain and Nervous Diseases
基金
国家自然科学基金青年基金项目(81801312)
河北省自然科学基金青年科学基金(H2018206233)
