摘要
目的探讨神经调节蛋白4(Nrg4)对2型糖尿病小鼠主动脉内皮细胞凋亡的影响。方法将C57小鼠分为对照(Vehicle)组、糖尿病(DM)组和Nrg4干预糖尿病小鼠(Nrg4)组。Nrg4组腹腔注射Nrg4重组蛋白(100μg/kg,3次/周),Vehicle组与DM组小鼠注射等量生理盐水,干预周期均为8周。实验结束后,TUNEL荧光染色检测主动脉内皮细胞凋亡,Western blot检测主动脉内皮组织凋亡相关蛋白Bax、Bcl-2及Cleaved-caspase 3的表达。结果与Vehicle组相比,DM组主动脉内皮细胞凋亡率明显升高[(33.0±5.4)%vs.(20.5±3.0)%;P<0.05],促凋亡蛋白Bax与凋亡执行蛋白Cleaved-caspase 3表达明显增多,抗凋亡蛋白Bcl-2表达显著减少(P<0.05);而Nrg4干预明显抑制糖尿病小鼠主动脉内皮细胞凋亡[(19.2±3.8)%vs.(33.0±5.4)%;P<0.05],降低Bax与Cleaved-caspase 3的表达并促进Bcl-2表达(P<0.05)。结论Nrg4干预能缓解2型糖尿病小鼠主动脉内皮细胞凋亡。
Objective To investigate the effects of neuregulin 4(Nrg4)on the apoptosis of endothelial cells in type 2 diabetic mice.Methods C57 mice were randomly divided into Vehicle group,diabetes mellites(DM)group and Nrg4 administration group(NRG4).Nrg4 group was intraperitoneally(i.p.)injected with Nrg4 recombinant protein(100μg/kg,3 times/week)and the same volume of saline was i.p.injected in the other two groups for 8 weeks.After the experiment,TUNEL staining was used to evaluate apoptotic rate of endothelial cells,and Western blot was used to investigate the protein levels of apoptosis-associated proteins.Results Compared with Vehicle group,DM significantly promoted cell apoptosis[(33.0±5.4)%vs.(20.5±3.0)%;P<0.05],increased Bax and cleaved-caspase 3 expression,and decreased Bcl-2 expression(P<0.05),while Nrg4 administration obviously decreased endothelial apoptosis[(19.2±3.8)%vs.(33.0±5.4)%;P<0.05],decreased Bax and cleaved-caspase 3 expression,and increased Bcl-2 expression(P<0.05).Conclusions Nrg4 administration can alleviate the apoptosis of aortic endothelial cells in type 2 diabetic mice.
作者
刘星宇
王莹
焦婷
方文灿
于汉英
张海松
LIU Xingyu;WANG Ying;JIAO Ting;FANG Wencan;YU Hanying;ZHANG Haisong(Department of General Medicine,Central Beijing Medical Sector of PLA General Hospital,Beijing 100120,China;PLA Medical School,Beijing 100853,China;Department of Medical Oncology,Affiliated Hospital of Hebei University/Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy,Baoding 071000,China)
出处
《武警医学》
CAS
2024年第7期593-596,601,共5页
Medical Journal of the Chinese People's Armed Police Force
基金
军队某课题资助
